Background: There is an increasing global demand for traditional medicines because of their efficacy, ease of availability, and few or no side effects. The Unani medicines have long been used in India and abroad for various disorders and diseases. Arqiyat (Arq; distillate) is one of the most widely used Unani formulation prepared by simple distillation from single or combination drugs. Objective: In the present investigation, an attempt has been made to generate data for quality control and stability testing of Arq formulations of the Unani Pharmacopeia of India that includes only three Arq formulations. Method: Arq-e-Nana (AeN), Arq-e-Gazar (AeG), and Arq-e-Brinjasif (AeB) were prepared through hydrodistillation. Quality control and stability analysis were performed by using HPTLC and GC-MS. Results: Dichloromethane extract of AeB showed maximum of 10 bands at different retardation factor values; however, there are 7 bands in AeN as well as in AeG. GC-MS analysis showed presence of 10 metabolites in AeG, 8 in AeN, and 9 in AeB. Stability studies showed that the shelf life of Arq formulations would be enhanced at refrigeration (5 ± 2°C) conditions. Conclusions: The present study highlights quality control and stability analysis of Arq formulations of Unani Pharmacopeia of India using HPTLC and GC-MS.

中文翻译：

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使用HPTLC和GC-MS对印度Unani药典的Arq制剂进行质量控制和稳定性测试。

背景：由于传统药物的功效，易获得性以及很少或没有副作用，因此全球对传统药物的需求不断增长。Unani药物长期以来在印度和国外用于治疗各种疾病。Arqiyat（Arq;馏出物）是最广泛使用的Unani制剂之一，可通过简单蒸馏从单一或组合药物中制备。目的：在本研究中，已尝试生成用于印度Unani药典中仅包含三种Arq制剂的Arq制剂的质量控制和稳定性测试的数据。方法：通过加氢蒸馏制备Arq-e-Nana（AeN），Arq-e-Gazar（AeG）和Arq-e-Brinjasif（AeB）。使用HPTLC和GC-MS进行质量控制和稳定性分析。结果： AeB的二氯甲烷萃取物在不同的延迟因子值下最多显示10条谱​​带。但是，在AeN和AeG中有7个频段。GC-MS分析显示，AeG中存在10种代谢产物，AeN中存在8种代谢产物，AeB中存在9种代谢产物。稳定性研究表明，在冷藏（5±2°C）条件下，Arq制剂的货架期将得到延长。结论：本研究重点介绍了使用HPTLC和GC-MS对印度Unani药典的Arq制剂进行质量控制和稳定性分析。