To assess the influence of unknown factors in endotoxemia, a conditioned medium, achieved by the stimulation of THP1 monocytes with lipopolysaccharide (LPS) [4h], was used for the stimulation of human umbilical vein endothelial cells (HUVECs) [16h]. SVEP1, KIAA0247, and SRPX2 were selected after microarray analysis. To study their possible functions, siRNAs of SVEP1, KIAA0247, or SRPX2 were used for the transfection of HUVECs and cells were stimulated with conditioned medium [16h]. Inhibition of SVEP1 expression resulted in an increase of soluble intercellular adhesion molecule (sICAM) 1 (10%) and soluble E-selectin (sE-selectin) (19%). Inhibition of SRPX2 led to an increase of sICAM (11%) and sE-selectin (14%). KIAA0247 negative HUVECs showed a decrease in monocyte chemoattractant protein (MCP) 1 of 16%. SVEP1 and SRPX2 seemed to act as regulators of ICAM1 and E-selectin shedding and influence the expression of membrane bound adhesion molecules.

中文翻译：

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内毒素血症体外细胞培养模型中SVEP1，KIAA和SRPX2的表征。

为了评估未知因素对内毒素血症的影响，使用通过脂多糖（LPS）刺激THP1单核细胞获得的条件培养基[4h]，来刺激人脐静脉内皮细胞（HUVECs）[16h]。微阵列分析后，选择了SVEP1，KIAA0247和SRPX2。为了研究其可能的功能，将SVEP1，KIAA0247或SRPX2的siRNA用于HUVEC的转染，并用条件培养基刺激细胞[16h]。SVEP1表达的抑制导致可溶性细胞间粘附分子（sICAM）1（10％）和可溶性E-选择素（sE-selectin）（19％）的增加。SRPX2的抑制导致sICAM（11％）和sE-选择素（14％）的增加。KIAA0247阴性HUVEC显示单核细胞趋化蛋白（MCP）1降低了16％。