The advent of proteomics techniques has been enthusiastically accepted in most areas of biology and medicine. In neuroscience, a host of applications was proposed ranging from neurotoxicology, neurometabolism, determination of the proteome of the individual brain areas in health and disease, to name a few. Only recently, the limitations of the method have been shown, hampering the rapid spreading of the technology, which in principle consists of two-dimensional gel electrophoresis with in-gel protein digestion of protein spots and identification by mass-spectrometrical approaches or microsequencing. The identification, including quantification using specific software, of brain protein classes, like enzymes, cytoskeleton proteins, heat shock proteins/chaperones, proteins of the transcription and translation machinery, synaptosomal proteins, antioxidant proteins, is a clear domain of proteomics. Furthermore, the concomitant detection of several hundred proteins on a gel allows the demonstration of an expressional pattern, rather generated by a reliable, protein-chemical method than by immunoreactivity, proposed by protein-arrays. An additional advantage is that hitherto unknown proteins, so far only proposed from their nucleic acid structure, designated as hypothetical proteins, can be identified as brain proteins. As to shortcomings and disadvantages of the method we would point to the major problem, the failure to separate hydrophobic proteins. There is so far no way to analyse the vast majority of these proteins in gels. Several other analytical problems need to be overcome, but once the latter problem can be solved, there is nothing to stop the method for a large scale analysis of membrane proteins in neuroscience.

中文翻译：

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脑研究中的蛋白质组学：潜力和局限性。

蛋白质组学技术的出现已在生物学和医学的大多数领域得到了热烈的接受。在神经科学领域，提出了许多应用，包括神经毒理学，神经代谢，确定健康和疾病中各个大脑区域的蛋白质组等等。仅在最近，该方法的局限性就受到了限制，阻碍了该技术的迅速推广，该技术原则上包括二维凝胶电泳和凝胶中蛋白质斑点的蛋白质消化，以及通过质谱方法或微测序进行鉴定。对脑蛋白类别的识别（包括使用特定软件进行的定量），包括脑酶类，酶，细胞骨架蛋白，热休克蛋白/伴侣蛋白，转录和翻译机制的蛋白，突触体蛋白，抗氧化剂蛋白质，是蛋白质组学的一个明确领域。此外，在凝胶上同时检测数百种蛋白质可以证明表达模式，而不是通过蛋白质阵列提出的可靠的蛋白质化学方法而非免疫反应性产生。另一个优点是，迄今仅从其核酸结构提出的迄今未知的蛋白质，称为假设蛋白质，可以鉴定为脑蛋白质。关于该方法的缺点和缺点，我们将指出主要问题，即无法分离疏水蛋白。到目前为止，还没有办法分析凝胶中的绝大多数这些蛋白质。还需要解决其他几个分析问题，但是一旦解决了后一个问题，