PHA synthase is a key enzyme for polyhydroxyalkanoate (PHA) production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that contain a class IV PHA synthase, such as some Bacillus spp. The phaR gene was developed to screen phaR-containing Bacillus spp. The phaR screening method contained two steps: phaR gene amplification by PCR and phaR amplicon detection using a DNA lateral flow assay. The screening method has a high specificity for phaR-containing Bacillus spp. The lowest amount of genomic DNA of B. thuringiensis ATCC 10792 that the phaR screening method could detect was 10 pg. This novel screening method improves the specificity and sensitivity of phaR gene screening and reduces the time and cost of the screening process, which could enhance the opportunity to discover good candidate PHA producers. Nevertheless, the screening method can certainly be used as a tool to screen phaR-containing Bacillus spp. from environmental samples.

中文翻译：

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一种用于筛选含有 phaR 的芽孢杆菌属的新型核横向流动测定法。


PHA 合酶是微生物生产聚羟基脂肪酸酯 (PHA) 的关键酶。 IV类PHA合酶由两个亚基组成：PhaC和PhaR。编码phaR基因的 PhaR 亚基仅存在于 IV 类 PHA 合酶中。因此， phaR基因被用作含有 IV 类 PHA 合酶的细菌（例如某些芽孢杆菌属）的生物标志物。开发phaR基因是为了筛选含有phaR的芽孢杆菌属。 phaR筛选方法包含两个步骤：通过PCR扩增phaR基因和使用DNA侧流测定法检测phaR扩增子。该筛选方法对含有phaR的芽孢杆菌属具有较高的特异性。 phaR筛选方法可以检测到的苏云金芽孢杆菌ATCC 10792基因组DNA的最低量为10 pg。这种新颖的筛选方法提高了phaR基因筛选的特异性和敏感性，并减少了筛选过程的时间和成本，这可以增加发现优秀候选 PHA 生产者的机会。尽管如此，该筛选方法当然可以用作筛选含有phaR的芽孢杆菌属的工具。来自环境样本。