Abundant evidence now supports the idea that methionine deficiency has negative effects on chicken healthy especially in the aspect of cell cycle regulation and apoptosis. But, lacking of knowledge is the evaluation on metabolic organs. To test the effect of methionine deficiency on the kidney, we assessed the apoptosis and the cell cycle of kidney induced by methionine deficiency by the methods of TdT-mediated dUTP Nick-End Labeling (TUNEL), flow cytometry (FCM), quantitative real-time PCR (qRT-PCR) and Enzyme-Linked ImmunoSorbent Assay (ELISA) of chickens for 42 days of age. Our results showed that the number of the apoptotic cells was increased (p < .05 or p < .01), while bcl-2 mRNA expression levels were decreased (p < .05 or p < .01) and bax and caspase-3 mRNA expression levels were higher (p < .01) in methionine deficiency group. Furthermore, the cell cycle results showed a time-dependent increase in G2M phase cells and a corresponding decrease in cells at G2M and S stages, the mRNA expression of p53 and p21 was increased (P < .05 or P < .01) and cyclin B and PCNA was significantly lower (P < .05 or P < .01) in the methionine deficiency group than that of the control group. These findings suggested that methionine deficiency could induce renal apoptosis and cell cycle arrest.

中文翻译：

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蛋氨酸缺乏对肉仔鸡肾脏细胞凋亡和细胞周期的影响。

现在有大量证据支持蛋氨酸缺乏对鸡肉健康有负面影响的观点，特别是在细胞周期调节和细胞凋亡方面。但是，缺乏知识是对代谢器官的评估。为了测试蛋氨酸缺乏对肾脏的影响，我们通过TdT介导的dUTP缺口末端标记（TUNEL），流式细胞术（FCM），定量实时分析方法评估了蛋氨酸缺乏诱导的肾脏的凋亡和细胞周期。鸡42天的实时PCR（qRT-PCR）和酶联免疫吸附测定（ELISA）。我们的结果表明，凋亡细胞的数量增加（p <.05或p <.01），而bcl-2 mRNA表达水平降低（p <.05或p <.01）以及bax和caspase-3 mRNA表达水平较高（p <。01）甲硫氨酸缺乏症组。此外，细胞周期结果显示，G2M期细胞的时间依赖性增加，G2M期和S期细胞相应减少，p53和p21的mRNA表达增加（P <.05或P <.01）和细胞周期蛋白蛋氨酸缺乏组的B和PCNA明显低于对照组（P <.05或P <.01）。这些发现表明蛋氨酸缺乏可以诱导肾细胞凋亡和细胞周期停滞。01）蛋氨酸缺乏症组比对照组少。这些发现表明蛋氨酸缺乏可以诱导肾细胞凋亡和细胞周期停滞。01）蛋氨酸缺乏症组比对照组少。这些发现表明蛋氨酸缺乏可以诱导肾细胞凋亡和细胞周期停滞。