Introduction: Protein microarray is a powerful tool for both biological study and clinical research. The most useful features of protein microarrays are their miniaturized size (low reagent and sample consumption), high sensitivity and their capability for parallel/high-throughput analysis. The major focus of this review is functional proteome microarray.

Areas covered: For proteome microarray, this review will discuss some recently constructed proteome microarrays and new concepts that have been used for constructing proteome microarrays and data interpretation in past few years, such as PAGES, M-NAPPA strategy, VirD technology, and the first protein microarray database. this review will summarize recent proteomic scale applications and address the limitations and future directions of proteome microarray technology.

Expert opinion: Proteome microarray is a powerful tool for basic biological and clinical research. It is expected to see improvements in the currently used proteome microarrays and the construction of more proteome microarrays for other species by using traditional strategies or novel concepts. It is anticipated that the maximum number of features on a single microarray and the number of possible applications will be increased, and the information that can be obtained from proteome microarray experiments will more in-depth in the future.

简介：蛋白质微阵列是生物学研究和临床研究的强大工具。蛋白质微阵列最有用的功能是它们的小型化（低试剂和样品消耗），高灵敏度及其并行/高通量分析能力。这篇综述的主要重点是功能蛋白质组芯片。

涵盖的领域：对于蛋白质组微阵列，本综述将讨论一些最近构建的蛋白质组微阵列以及在过去几年中用于构建蛋白质组微阵列和数据解释的新概念，例如PAGES，M-NAPPA策略，VirD技术以及第一个蛋白质微阵列数据库。这篇综述将总结蛋白质组学的最新应用，并探讨蛋白质组微阵列技术的局限性和未来发展方向。

专家意见：蛋白质组微阵列是用于基础生物学和临床研究的强大工具。通过使用传统策略或新概念，预期将看到当前使用的蛋白质组微阵列的改进以及为其他物种构建更多的蛋白质组微阵列。预计单个微阵列上的最大特征数量和可能的应用数量将增加，并且将来可以从蛋白质组学微阵列实验中获得的信息将更加深入。