ABSTRACT To investigate the effects of maternal 25-hydroxycholecalciferol (25OHD3) supplementation during lactation on nutrient digestibility and milk composition of sows and gut bacterial metabolites and their metabolites in the hindgut of suckling piglets, 24 Large White × Landrace sows were assigned randomly to one of two dietary treatments (Diet ND: 2000 IU vitamin D3/kg feed; Diet 25-D: 50 μg 25OHD3/kg feed). The experiment began on d 107 of gestation and continued until weaning on d 21 of lactation. Maternal 25OHD3 supplementation increased (p < 0.05) total litter weight gain during lactation. Milk fat content, immunoglobulin G level on d 21 of lactation and 25OHD3 concentration on d 7, 14, and 21 of lactation were higher (p < 0.05) in sows fed with 25OHD3. Apparent total tract digestibility of dietary calcium was higher (p < 0.05) in 25-D sows than ND sows. With respect to fatty-acid profile, C16:0 and saturated fatty acids in milk were higher (p < 0.05), but C20:4n-6, the ratios of monounsaturated fatty acids to saturated fatty acids and polyunsaturated fatty acids to saturated fatty acids were lower (p < 0.05) in 25-D sows than ND sows. 25OHD3 supplementation increased the mRNA expressions of acetyl-CoA carboxylase α and fatty-acid synthase in the mammary gland of lactating sows. For gut bacterial metabolites, concentration of butyrate in the caecal digesta was higher (p < 0.05) in piglets suckling 25-D sows than piglets suckling ND sows. In conclusion, 25OHD3 supplementation in maternal diets changed dietary calcium digestibility, milk composition and milk fatty-acid profile of lactating sows and altered gut bacterial metabolites in the hindgut of suckling piglets.

中文翻译：

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母猪 25-羟基胆钙化醇对哺乳母猪营养消化率、乳汁成分和脂肪酸组成以及哺乳仔猪后肠肠道细菌代谢物的影响

摘要 为了研究哺乳期母猪补充 25-羟基胆钙化醇 (25OHD3) 对母猪营养消化率和牛奶成分以及哺乳仔猪后肠中肠道细菌代谢物及其代谢物的影响，24 头大白 × 长白母猪被随机分配到其中一头母猪。两种日粮处理（日粮 ND：2000 IU 维生素 D3/kg 饲料；日粮 25-D：50 μg 25OHD3/kg 饲料）。实验从妊娠第 107 天开始，一直持续到泌乳第 21 天断奶。在哺乳期间，母体 25OHD3 补充剂增加（p < 0.05）总窝增重。饲喂 25OHD3 的母猪的乳脂含量、泌乳第 21 天的免疫球蛋白 G 水平和泌乳第 7、14 和 21 天的 25OHD3 浓度较高（p < 0.05）。膳食钙的表观总消化率更高（p < 0. 05) 在 25-D 母猪中比 ND 母猪。在脂肪酸谱方面，牛奶中的 C16:0 和饱和脂肪酸较高 (p < 0.05)，但 C20:4n-6，单不饱和脂肪酸与饱和脂肪酸和多不饱和脂肪酸与饱和脂肪酸的比率25-D 母猪比 ND 母猪低 (p < 0.05)。补充25OHD3增加了泌乳母猪乳腺中乙酰辅酶A羧化酶α和脂肪酸合酶的mRNA表达。对于肠道细菌代谢物，哺乳 25 日龄母猪的小猪盲肠食糜中丁酸盐的浓度高于哺乳 ND 母猪的仔猪（p < 0.05）。总之，在母体饮食中补充 25OHD3 改变了饮食钙的消化率，