Abstract Point mutations are well characterized activators of oncogenes but are often indistinguishable using common gene technologies. In general, the precise sites of point-mutated oncogenes are difficult to distinguish under physiological conditions primarily because single nucleotide mismatch do not affect the annealing temperatures of DNA probes sufficiently. To address this limitation, we developed photo-responsive oligodeoxyribonucleotides containing 2′-O-[N-(4,5′,8-trimethylpsoralen-4'-ylmethylcarbamoyl)]adenosine (Ps-amd-Oligo), which can be used to selectively manipulate and identify genes with point mutations. Here we present time course analyses of the photo-cross-linking efficiency of Ps-amd-Oligo with DNA and RNA and show promising selectivity for the oncogene H-ras.

中文翻译：

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含有 2'-O-[N-(4,5',8-trimethylpsoralen-4'-ylmethylcarbamamyl)]腺苷的寡脱氧核糖核苷酸对突变 H-ras DNA 的选择性交联行为

摘要 点突变是致癌基因的充分表征的激活因子，但使用常见的基因技术通常无法区分。一般来说，点突变癌基因的精确位点在生理条件下很难区分，主要是因为单核苷酸错配不会充分影响 DNA 探针的退火温度。为了解决这一限制，我们开发了含有 2'-O-[N-(4,5',8-trimethylpsoralen-4'-ylmethylcarbamoyl)]腺苷 (Ps-amd-Oligo) 的光响应寡脱氧核糖核苷酸，可用于选择性地操纵和识别具有点突变的基因。在这里，我们展示了 Ps-amd-Oligo 与 DNA 和 RNA 的光交联效率的时间进程分析，并显示了对致癌基因 H-ras 的选择性。