The use of herbs as medicine is an ancient form of healthcare known to mankind. Standardization of herbal medicines is however a challenging task and is the major bottleneck in their acceptance as the primary therapeutic option. The aim of this study was to develop and validate a simple, rapid HPLC method for standardizing the mixture of extracts of three Medhya Rasayanas (neurotonic), Convolvulus pluricaulis, Withania somnifera and Bacopa monnieri. Simultaneous estimation of the respective bioactive markers of these plants viz., scopoletin, withaferin A, bacoside A 3, bacopaside II, jujubogenin and bacosaponin C has been reported for the first time. The method was developed using Waters Hybrid X-Bridge shield with BEH technology 2.5 μm, 4.6 × 75 mm column and validated according to ICH guidelines. The 20 minutes run time makes the method eco-friendly. The method was linear over a range of 12.5-400 ng/10 μL for scopoletin and 62.5-2,000 ng/10 μL for withaferin A, bacoside A 3, bacopaside II, jujubogenin and bacosaponin C with detection limits of 8.0, 48.3, 30.4, 40.7, 15.6 and 18.9 ng/10 μL and quantification limits of 24.5, 146.5, 92.2, 123.4, 47.4 and 57.4 ng/10 μL, respectively. The correlation coefficient for each analyte was >0.999. The intra-day and inter-day precision was <2%. These results confirmed the precision, accuracy and robustness of the proposed method.

中文翻译：

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同时测定空心菜，Withania睡茄和Bacopa monnieri提取物混合物中生物活性标记化合物的新型快速梯度HPLC方法的开发和验证。

使用草药作为药物是人类已知的一种古老的医疗保健形式。然而，草药的标准化是一项艰巨的任务，并且是其被接受为主要治疗选择的主要瓶颈。这项研究的目的是开发和验证一种简单，快速的HPLC方法，以标准化三种Medhya Rasayanas（中旋子），Convolvulus pluricaulis，Withania somnifera和Bacopa monnieri提取物的混合物。首次报道了同时估算这些植物的生物活性标记的方法，即大麦草碱，枯草杆菌素A，巴可糖苷A 3，bacopaside II，大枣生成素和bacosaponinC。该方法是使用具有2.5μmBEH技术，4.6×75 mm色谱柱的Waters Hybrid X-Bridge防护罩开发的，并根据ICH指南进行了验证。20分钟的运行时间使该方法变得环保。该方法在scopoletin 12.5-400 ng / 10μL和aferin A，bacoside A 3，bacopaside II，jujubogenin和bacosaponin C的62.5-2,000 ng / 10μL范围内是线性的，检出限为8.0、48.3、30.4，分别为40.7、15.6和18.9 ng / 10μL，定量限分别为24.5、146.5、92.2、123.4、47.4和57.4 ng / 10μL。每种分析物的相关系数> 0.999。日内和日间精确度<2％。这些结果证实了该方法的准确性，准确性和鲁棒性。9 ng / 10μL，定量限分别为24.5、146.5、92.2、123.4、47.4和57.4 ng / 10μL。每种分析物的相关系数> 0.999。日内和日间精确度<2％。这些结果证实了该方法的准确性，准确性和鲁棒性。9 ng / 10μL，定量限分别为24.5、146.5、92.2、123.4、47.4和57.4 ng / 10μL。每种分析物的相关系数均大于0.999。日内和日间精确度<2％。这些结果证实了该方法的准确性，准确性和鲁棒性。