Angiogenesis in adipose tissue is promoted by insulin-like growth factor 1 signaling. We analyzed whether this regulatory mechanism also improves the angiogenic activity of adipose tissue-derived microvascular fragments. Murine adipose tissue-derived microvascular fragments were cultivated for 24 h in the University of Wisconsin (UW) solution supplemented with vehicle, insulin-like growth factor 1, or a combination of insulin-like growth factor 1 and insulin-like growth factor-binding protein 4. Subsequently, we assessed their cellular composition, viability, proliferation, and growth factor expression. Moreover, cultivated adipose tissue-derived microvascular fragments were seeded onto collagen-glycosaminoglycan scaffolds, which were implanted into dorsal skinfold chambers to study their vascularization and incorporation. Insulin-like growth factor 1 increased the viability and growth factor expression of adipose tissue-derived microvascular fragments without affecting their cellular composition and proliferation. Accordingly, scaffolds containing insulin-like growth factor 1-stimulated adipose tissue-derived microvascular fragments exhibited an enhanced in vivo vascularization and incorporation. These positive insulin-like growth factor 1 effects were reversed by additional exposure of adipose tissue-derived microvascular fragments to insulin-like growth factor-binding protein 4. Our findings indicate that insulin-like growth factor 1 stimulation of adipose tissue-derived microvascular fragments is suitable to improve their vascularization capacity.

中文翻译：

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胰岛素样生长因子1刺激脂肪组织衍生的微血管片段的血管生成活性。

胰岛素样生长因子1信号传导促进脂肪组织中的血管生成。我们分析了这种调节机制是否也改善了脂肪组织衍生的微血管片段的血管生成活性。在补充了媒介物，胰岛素样生长因子1或胰岛素样生长因子1和胰岛素样生长因子结合的补充的威斯康星大学（UW）溶液中培养小鼠脂肪组织衍生的微血管片段24小时蛋白4。随后，我们评估了它们的细胞组成，生存力，增殖和生长因子表达。此外，将培养的源自脂肪组织的微血管片段播种到胶原蛋白-糖胺聚糖支架上，然后将其植入背部皮褶腔中以研究其血管形成和整合。胰岛素样生长因子1增加了脂肪组织衍生的微血管片段的活力和生长因子表达，而没有影响它们的细胞组成和增殖。因此，包含胰岛素样生长因子1刺激的脂肪组织衍生的微血管片段的支架表现出增强的体内血管形成和掺入。通过将脂肪组织衍生的微血管片段额外暴露于胰岛素样生长因子结合蛋白4，可以逆转这些胰岛素样生长因子1阳性效应。我们的研究结果表明，胰岛素样生长因子1刺激脂肪组织衍生的微血管片段适合提高其血管化能力。