Background: Studies have shown that consumption of high levels of alcohol causes many negative effects on the liver and kidneys where antioxidant ingredients can be a proper solution to reducing the resulting damages. So, the present study investigated the effect of hydroalcoholic extract of Crocus sativus L. (saffron) petal with antioxidant properties on the changes in inflammatory and enzymatic indices resulting from alcohol use in the male rats’ kidney and liver.
Materials and methods: After preparing the extract, LD₅₀ was determined and high-performance liquid chromatography (HPLC) was employed to specify the type and the rate of the active ingredients of the extract. Then, 36 male Wistar rats were randomly assigned into six groups (n=6). The first group was only administered with normal saline and the second group only received ethyl alcohol 6 mL/kg/day·BW. The third and the fourth groups received ethyl alcohol 6 mL/kg/day·BW plus 167.5 and 335 mg/kg/day·BW saffron petal extract for 8 weeks. The fifth and the sixth groups received ethyl alcohol 6 mL/kg/day·BW for the first 8 weeks and were subsequently gavage fed on saffron extract for 167.5 and 335 mg/kg/day·BW, respectively, during the next 8 weeks. In the beginning and after the termination of the treatment, blood samples were collected from all rats.
Results: The LD₅₀ of the extract was about 670 mg/kg. The HPLC results indicated that the extract contains important antioxidant ingredients. At the end of the study, the serum concentration of the inflammatory indices, renal enzymes, and hepatic enzymes experienced a significant reduction in all of the intervened groups compared to the negative control group (minimum significant difference: P<0.05) except for the treatment group 1.
Conclusion: Based on the current results, the extract has a protective effect in a dosage-dependent way and greater protective roles were documented for higher dosages.



中文翻译：

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研究番红花水醇提取物对雄性大鼠肾脏和肝脏酒精使用引起的炎症和酶学指标的影响。

背景：研究表明，大量饮酒会对肝脏和肾脏造成许多负面影响，其中抗氧化成分可以成为减少由此产生的损害的适当解决方案。因此，本研究调查了具有抗氧化特性的番红花花瓣水醇提取物对雄性大鼠肾脏和肝脏因饮酒引起的炎症和酶学指标变化的影响。
材料和方法：制备提取物后，LD ₅₀测定并采用高效液相色谱法 (HPLC) 确定提取物活性成分的类型和比例。然后，将 36 只雄性 Wistar 大鼠随机分为 6 组（n=6）。第一组仅给予生理盐水，第二组仅给予乙醇6 mL/kg/day·BW。第三组和第四组分别给予乙醇6 mL/kg/day·BW加167.5和335 mg/kg/day·BW藏红花花瓣提取物8周。第五组和第六组在前 8 周接受 6 mL/kg/day·BW 的乙醇，随后在接下来的 8 周内分别灌胃藏红花提取物 167.5 和 335 mg/kg/day·BW。在治疗开始和结束后，从所有大鼠中采集血样。
结果： LD₅₀的提取物约为 670 mg/kg。HPLC结果表明提取物含有重要的抗氧化成分。研究结束时，除治疗组外，所有干预组的血清炎症指标、肾酶和肝酶浓度均较阴性对照组显着降低（最小显着性差异：P <0.05）第1组。
结论：根据目前的结果，提取物具有剂量依赖性的保护作用，并且记录了更高剂量的更大保护作用。