Anaerobic cultivable microbial communities in thermal springs producing hydrolytic enzymes were studied. Thermal water samples from seven thermal springs located in the Andean volcanic belt, in the eastern and central mountain ranges of the Colombian Andes were used as inocula for the growth and isolation of thermophilic microorganisms using substrates such as starch, gelatin, xylan, cellulose, Tween 80, olive oil, peptone and casamino acids. These springs differed in temperature (50–70 °C) and pH (6.5–7.5). The predominant ion in eastern mountain range thermal springs was sulphate, whereas that in central mountain range springs was bicarbonate. A total of 40 anaerobic thermophilic bacterial strains that belonged to the genera Thermoanaerobacter, Caloramator, Anoxybacillus, Caloranaerobacter, Desulfomicrobium, Geotoga, Hydrogenophilus, Desulfacinum and Thermoanaerobacterium were isolated. To investigate the metabolic potential of these isolates, selected strains were analysed for enzymatic activities to identify strains than can produce hydrolytic enzymes. We demonstrated that these thermal springs contained diverse microbial populations of anaerobic thermophilic comprising different metabolic groups of bacteria including strains belonging to the genera Thermoanaerobacter, Caloramator, Anoxybacillus, Caloranaerobacter, Desulfomicrobium, Geotoga, Hydrogenophilus, Desulfacinum and Thermoanaerobacterium with amylases, proteases, lipases, esterases, xylanases and pectinases; therefore, the strains represent a promising source of enzymes with biotechnological potential.

中文翻译：

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哥伦比亚安第斯温泉：产生水解酶的嗜热厌氧细菌的储水池。

研究了产生水解酶的温泉中厌氧菌的微生物群落。来自位于哥伦比亚安第斯山脉东部和中部山脉安第斯火山带的七个温泉的温泉水样本被用作接种物，用于利用淀粉，明胶，木聚糖，纤维素，吐温等底物生长和分离嗜热微生物80，橄榄油，蛋白ept和酪蛋白氨基酸。这些弹簧的温度（50–70°C）和pH（6.5–7.5）不同。东部山区温泉的主要离子为硫酸根，而中部山区温泉的主要离子为碳酸氢根。属于属的共40个厌氧嗜热细菌菌株分离了嗜热厌氧杆菌，嗜热嗜热杆菌，厌氧杆菌，嗜热厌氧杆菌，脱硫微生物，地线藻，嗜氢菌，脱硫球菌和嗜热厌氧杆菌。为了研究这些分离物的代谢潜力，分析了所选菌株的酶活性，以鉴定可产生水解酶的菌株。我们表明，这些温泉所含的厌氧嗜热多样的微生物种群，其包括细菌，包括属于下列属的菌株的不同的代谢组嗜热，Caloramator，Anoxybacillus，Caloranaerobacter，Desulfomicrobium，Geotoga，Hydrogenophilus，Desulfacinum和Thermoanaerobacterium带有淀粉酶，蛋白酶，脂肪酶，酯酶，木聚糖酶和果胶酶；因此，菌株代表了具有生物技术潜力的酶的有希望的来源。