We previously showed that deregulation of PEG3 domain genes is associated with intrauterine growth restriction (IUGR) in cattle. Here, we carried out genome-wide DNA methylation analysis of foetal placenta in two IUGR and wild-type samples and identified a differentially methylated region (DMR) in intron 2 of MIMT1. Pyrosequencing on a larger sample size (n = 20) confirmed significantly lower (P < 0.001) MIMT1-DMR methylation in foetal and maternal placenta of IUGR than wild-type conceptuses. Our study demonstrates that small-scale whole-genome bisulphite sequencing can be used to identify epigenetic regulatory elements in a defined genome locus.

中文翻译：

---

小型全基因组甲基化测序检测到牛MIMT1中的甲基化差异区域。

我们先前显示，PEG3域基因的失控与牛的子宫内生长限制（IUGR）相关。在这里，我们对两个IUGR和野生型样品中的胎盘进行了全基因组DNA甲基化分析，并在MIMT1的内含子2中鉴定了差异甲基化区域（DMR）。在较大样本量（n  = 20）上进行焦磷酸测序证实，IUGR的胎儿和孕妇胎盘中的MIMT1- DMR甲基化程度明显低于野生型概念（P  <0.001）。我们的研究表明，小规模的全基因组亚硫酸氢盐测序可用于鉴定已定义的基因组基因座中的表观遗传调控元件。