Aminotransferases have attracted considerable attention due to their extraordinary potential for the biosynthesis of chiral amines. Research on transaminase genes can facilitate their application to various fields. Herein, 89 putative aminotransferase genes potentially encoding useful biocatalysts were identified in three Bacillus strains genomes by genome annotation. Enzymes encoded by genes ota3, ota8, otae6, otae21, otaf1, otaf8, and otaf26 belong to pyridoxine 5′-phosphate-dependent enzyme class IV. These seven ω-aminotransferase genes are highly conserved according to phylogenetic tree and bioinformatics analyses, as are the putative lysine catalytic residues in the corresponding enzymes (ω-BPTA 1–7). The enzymes may possess similar activity to ω-aminotransferases from Arthrobacter sp. KNK 168. The potential application of these novel enzymes for the synthesis of medicinal amino compounds will be explored in future genetic engineering studies.

中文翻译：

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通过基因组分析和生物信息学比较三种芽孢杆菌枯草芽孢杆菌W3，velezensis芽孢杆菌SYBC H47和解淀粉芽孢杆菌YP6的氨基转移酶。

氨基转移酶由于其手性胺生物合成的巨大潜力而​​备受关注。转氨酶基因的研究可以促进其在各个领域的应用。在本文中，通过基因组注释在三个芽孢杆菌菌株的基因组中鉴定了潜在编码有用的生物催化剂的89个推定的氨基转移酶基因。由ota3，ota8，otae6，otae21，otaf1，otaf8和otaf26基因编码的酶属于吡ido醇5'-磷酸盐依赖性酶IV类。根据系统进化树和生物信息学分析，这七个ω-氨基转移酶基因高度保守，相应酶中的假定赖氨酸催化残基也是如此（ω-BPTA1-7）。这些酶可能具有与节杆菌属细菌的ω-氨基转移酶相似的活性。KNK 168.在今后的基因工程研究中将探讨这些新型酶在合成药用氨基化合物中的潜在应用。