Mastitis, an inflammatory response of mammary glands to invading bacteria, is one of the most economically costly diseases affecting dairy animals. Escherichia coli can be introduced as a major etiological agent of bovine mastitis in well-managed dairy farms. It is of great significance to understand the regulatory mechanisms by which the disease can be controlled. High-throughput technologies combined with novel computational systems biology tools have provided new opportunities for a better understanding of the molecular mechanisms that underlie disease. In the current study, the results of microarray meta-analysis research were used to perform a network analysis to potentially identify molecular mechanisms that regulate gene expression profile in response to E. coli mastitis. In our result, transcription factors, TP53, SP1, ligands, INS, IFNG, EGF, and protein kinases, MAPK1, MAPK14, AKT1, were identified as the key upstream regulators whereas protein kinases, MAPK3, MAPK8, MAPK14, ligands, VEGFA, IL10, an extracellular protein, MMP2, and a mitochondrial membrane protein, BCL2, were identified as the key downstream targets of differentially expressed genes. The results of this research revealed important genes that have the key functions in immune response, inflammation, or mastitis which can provide the basis for strategies to improve the diagnosis and treatment of mastitis in dairy cows.

中文翻译：

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大肠杆菌诱导的乳腺炎的关键调控因子和下游靶点的预测。

乳腺炎是乳腺对入侵细菌的炎性反应，是影响奶牛的最经济昂贵的疾病之一。在管理良好的奶牛场中，大肠杆菌可以作为牛乳腺炎的主要病原体。了解可以控制疾病的调节机制具有重要意义。高通量技术与新颖的计算系统生物学工具相结合，为更好地理解构成疾病基础的分子机制提供了新的机会。在当前的研究中，微阵列荟萃分析研究的结果用于进行网络分析，以潜在地识别调节基因表达谱以响应大肠杆菌的分子机制。乳腺炎。在我们的结果中，转录因子TP53，SP1，配体，INS，IFNG，EGF和蛋白激酶MAPK1，MAPK14，AKT1被确定为关键的上游调节子，而蛋白激酶MAPK3，MAPK8，MAPK14，配体，VEGFA，IL10，细胞外蛋白MMP2和线粒体膜蛋白BCL2被鉴定为差异表达基因的关键下游靶标。这项研究的结果揭示了在免疫反应，炎症或乳腺炎中具有关键功能的重要基因，这些基因可以为提高乳牛乳腺炎的诊断和治疗策略提供基础。