The present study investigated the effects of citrinin (CIT) on a yeast-transformed human DNA methyltransferase (DNMT) associated with flocculation that can be inhibited by epigenetic mutagens. CIT (0.5–2 μmol/L) inhibited the flocculation levels of yeast transfected with DNMT-genes (DNMT yeast) and the reporter gene activity of FLO1, which has been associated with flocculation. In contrast, the same concentrations of CIT had little effect on reporter activity under the control of a less methylation-sensitive FLO1 promoter. It was also shown that bacterial DNMT activity could be inhibited in the presence of CIT (4 and 40 μmol/L). These results show that CIT has inhibitory activity of DNMT, suggesting that the cytotoxicity of CIT may be involved in epigenetic mutagenicity.

中文翻译：

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使用基于酵母的生物测定法检测柠檬绿素的表观遗传学作用。

本研究调查了柑桔素（CIT）对酵母转化的人类DNA甲基转移酶（DNMT）的影响，该酶与絮凝作用有关，而絮凝作用可被表观诱变剂抑制。CIT（0.5-2微摩尔/ L）抑制与转染的酵母的絮凝水平DNMT -genes（DNMT酵母）和报告基因的活性FLO1，其已经用絮凝相关联。相反，在甲基化敏感性较低的FLO1的控制下，相同浓度的CIT对报道分子活性几乎没有影响。启动子。还显示在CIT（4和40μmol/ L）存在下，细菌DNMT活性可以被抑制。这些结果表明，CIT具有DNMT的抑制活性，表明CIT的细胞毒性可能与表观遗传诱变有关。