Glioma, as one of the most aggressive tumors, is hardly cleaned by surgical removal, leading to a low survival rate. m6A is an internal modification in RNA and plays an important role in many kinds of cancers. In our study, we detected that the m6A level was decreased in glioma tissue, which might be caused by decreased METTL3 and increased FTO levels. We upregulated the m6A level in U251 cells by overexpressing METTL3. The results showed that a high level of m6A led to a reduced migration and proliferation ability, and vice versa. Finally, we performed a TUNEL assay and showed that m6A regulated cell proliferation by influencing apoptosis of U251 cells through regulating HSP90 expression.

中文翻译：

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m6A RNA 甲基化通过影响细胞凋亡控制人神经胶质瘤细胞的增殖

胶质瘤作为最具侵袭性的肿瘤之一，很难通过手术切除来清除，导致存活率低。m6A 是 RNA 的内部修饰，在多种癌症中发挥重要作用。在我们的研究中，我们检测到胶质瘤组织中 m6A 水平降低，这可能是由 METTL3 降低和 FTO 水平升高引起的。我们通过过表达 METTL3 上调了 U251 细胞中的 m6A 水平。结果表明，高水平的 m6A 导致迁移和增殖能力降低，反之亦然。最后，我们进行了 TUNEL 测定，结果表明 m6A 通过调节 HSP90 表达影响 U251 细胞的凋亡来调节细胞增殖。