Fluorescence acquisition and image display over a high dynamic range is highly desirable. However, the limited dynamic range of current photodetectors and imaging charge-coupled devices impose a limit on the fluorescence intensities that can be simultaneously captured during a single image acquisition. This is particularly troublesome when imaging biological samples, where protein expression fluctuates considerably. As a result, biological images will often contain regions with signal that is either saturated or hidden within background noise, causing information loss. In this paper, we summarize recent work from our group and others, to extended conventional to high dynamic range fluorescence imaging. These strategies have many biological applications, such as mapping of neural connections, vascular imaging, bio-distribution studies or pharmacologic imaging at the single cell and organ level.

中文翻译：

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高动态范围荧光成像

非常需要高动态范围内的荧光采集和图像显示。然而，当前光电探测器和成像电荷耦合器件的有限动态范围对在单个图像采集期间可以同时捕获的荧光强度施加了限制。这在对生物样品进行成像时尤其麻烦，因为蛋白质表达波动很大。因此，生物图像通常会包含信号饱和或隐藏在背景噪声中的区域，从而导致信息丢失。在本文中，我们总结了我们小组和其他人最近的工作，将常规荧光成像扩展到高动态范围荧光成像。这些策略有许多生物学应用，例如神经连接映射、血管成像、