Fatty liver is common in men and post-menopausal women, suggesting that estrogen may be involved in liver lipid metabolism. The aim of this study is to be clear the role of estrogen and estrogen receptor alpha (ERα) in fat accumulation during liver regeneration using the 70% partial hepatectomy (PHX) model in male, female, ovariectomized (OVX) and E2-treated OVX (OVX-E2) rats. Liver tissues were sampled at 0-48 hr after PHX and fat accumulation, fatty acid translocase (FAT/CD36), sterol regulatory element-binding protein (SREBP1c), peroxisome proliferator-activated receptor α (PPARα), proliferative cell nuclear antigen (PCNA) and ERα were examined by Oil Red O, qRT-PCR and immunohistochemistry, respectively. Hepatic fat accumulation was abundant in female and OVX-E2 compared to male and OVX rats. FAT/CD36 expression was observed in female, OVX and OVX-E2 at 0-12 hr after PHX, but not in male rats. At 0 hr, SREBP1c and PPARα were elevated in female and male rats, respectively, but were decreased after PHX in all rats. The PCNA labeling index reached a maximum at 36 hr and 48 hr in OVX-E2 and OVX rats, respectively. ERα expression in OVX-E2 was higher than OVX at 0-36 hr after PHX. In conclusion, these results indicated that estrogen and ERα might play an important role in fat accumulation related to FAT/CD36 during early phase of rat liver regeneration.

中文翻译：

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雌激素对大鼠部分肝切除术后肝再生早期肝脏脂肪积累的影响。

脂肪肝在男性和绝经后女性中很常见，这表明雌激素可能与肝脏脂质代谢有关。这项研究的目的是使用70％部分肝切除术（PHX）模型在男性，女性，卵巢切除（OVX）和E2治疗的OVX中明确雌激素和雌激素受体α（ERα）在肝脏再生过程中脂肪积累中的作用（OVX-E2）大鼠。在PHX和脂肪蓄积后0-48小时取样肝组织，脂肪酸转位酶（FAT / CD36），固醇调节元件结合蛋白（SREBP1c），过氧化物酶体增殖物激活受体α（PPARα），增殖细胞核抗原（PCNA） ）和ERα分别通过Oil Red O，qRT-PCR和免疫组织化学检查。与雄性和OVX大鼠相比，雌性和OVX-E2的肝脂肪积累丰富。在女性中观察到FAT / CD36表达，PHX后0-12小时的OVX和OVX-E2，但雄性大鼠没有。在0小时时，雌性和雄性大鼠中的SREBP1c和PPARα分别升高，但所有大鼠在PHX后均降低。在OVX-E2和OVX大鼠中，PCNA标记指数分别在36小时和48小时达到最大值。在PHX后0-36小时，OVX-E2中的ERα表达高于OVX。总之，这些结果表明雌激素和ERα可能在大鼠肝再生的早期阶段与FAT / CD36相关的脂肪蓄积中起重要作用。在PHX后0-36小时，OVX-E2中的ERα表达高于OVX。总之，这些结果表明雌激素和ERα可能在大鼠肝再生的早期阶段与FAT / CD36相关的脂肪蓄积中起重要作用。在PHX后0-36小时，OVX-E2中的ERα表达高于OVX。总之，这些结果表明雌激素和ERα可能在大鼠肝再生的早期阶段与FAT / CD36相关的脂肪蓄积中起重要作用。