Corneal decellularization represents a promising alternative source of human donor with global shortage. Multiple methods have been developed for the preparation of decellularized porcine corneal stroma. However, most strategies relied on long-time treatment to facilitate the entry of detergents or nucleases, which may cause irreversible ultrastructural damage. Here, we developed a rapid decellularization method for porcine corneal stroma through the combined mild detergent sodium N-lauroyl glutamate (SLG) and supernuclease. Compared with traditional methods, the novel decellularization method allowed the efficient removal of xenoantigen DNA within 3 h, while retaining the ultrastructure, transparency, and mechanical properties of porcine corneas. When transplanted in rabbit model for 1 month, the decellularized porcine corneal grafts presented favorable transparency and biocompatibility without immune rejection. Therefore, the combined use of detergent SLG and supernuclease may serve as a promising method for the clinical use of decellularized porcine cornea.

中文翻译：

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通过使用N-月桂酰谷氨酸钠和超核酸酶快速进行猪角膜脱细胞。

角膜脱细胞代表了全球短缺的人类供体的有希望的替代来源。已经开发出多种方法来制备脱细胞的猪角膜基质。但是，大多数策略都依靠长期处理来促进去污剂或核酸酶的进入，这可能会导致不可逆的超微结构破坏。在这里，我们通过结合温和的去污剂N-月桂酰谷氨酸钠（SLG）和超核酸酶开发了一种猪角膜基质的快速脱细胞方法。与传统方法相比，新的脱细胞方法可以在3小时内有效去除异种抗原DNA，同时保留猪角膜的超微结构，透明性和机械性能。在兔子模型中移植1个月后，去细胞猪角膜移植物具有良好的透明性和生物相容性，没有免疫排斥反应。因此，洗涤剂SLG和超核酸酶的组合使用可作为临床应用去细胞猪角膜的一种有前途的方法。