Abstract Introduction A comprehensive description is presented of four novel cases ofamorphus globosus (ag) foetuses originating from multiple pregnancies of Polish Holstein cows. Material and Methods Four amorphic foetuses were delivered by three cows. Tissue samples were collected during autopsy, embedded in paraffin, sectioned, and stained with haematoxylin and eosin. Genomic DNA was isolated from tissue samples of abnormal foetuses and from blood leukocytes of their healthy siblings. PCR reactions were used to reveal the presence of Y-linked genes (SRY and AMELY) and an X-linked gene (AMELX). Results All foetuses were classified to the groupholoacardius amorphous (anideus). Molecular analysis clearly showed that at 17 microsatellite loci, the studied amorphous foetuses had identical genotypes to the viable co-twins. Conclusion Foetuses had monozygotic origin. Histological analysis showed a low level of development of tissues of meso- and ectodermal origin, as well as features of degrading patterns.

中文翻译：

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波兰荷斯坦牛的球状魔芋：解剖学、组织学和遗传学研究

摘要 介绍 对来自波兰荷斯坦奶牛多胎妊娠的 4 个新的球状紫晶 (ag) 胎儿病例进行了全面的描述。材料与方法 三头奶牛分娩了四个无定形胎儿。在尸检期间收集组织样本，包埋在石蜡中，切片，并用苏木精和伊红染色。从异常胎儿的组织样本和健康兄弟姐妹的血液白细胞中分离出基因组 DNA。PCR 反应用于揭示 Y 连锁基因（SRY 和 AMELY）和 X 连锁基因（AMELX）的存在。结果所有胎儿均被归类为无定形心全组（anideus）。分子分析清楚地表明，在 17 个微卫星位点，所研究的无定形胎儿与可存活的双胞胎具有相同的基因型。结论 胎儿为单卵来源。组织学分析显示中胚层和外胚层起源的组织发育水平低，以及退化模式的特征。