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Bivagina pagrosomi Murray (1931) (Monogenea: Polyopisthocotylea), a microcotylid infecting the gills of the gilt-head sea bream Sparus aurata (Sparidae) from the Red Sea: morphology and phylogeny
Journal of Veterinary Research ( IF 1.8 ) Pub Date : 2019-09-13 , DOI: 10.2478/jvetres-2019-0053
Saad Bin Dajem 1 , Kareem Morsy 1, 2 , Ali Shati 1 , Ahmed Ezzat 1, 3 , Fahmy El-Said 1, 4 , Rewaida Abdel-Gaber 2, 5
Affiliation  

Abstract Introduction Monogenea is a class of ectoparasitic flatworms on the skin, gills, or fins of fish. Microcotylidae is a family of polyopisthocotylean monogeneans parasitising only marine fishes. This work describes and taxonomically determines a microcotylid polyopisthocotylean monogenean in an important fish in Saudi aquaculture. Material and Methods Thirty gilt-head sea bream captured alive from the Red Sea of Saudi Arabia were examined for monogenean infection. Worms were described morphologically and morphometrically by light microscopy and multiple sequence alignments and phylogenetic trees were also constructed after maximum likelihood analysis of the 28S rRNA sequences. Results Seventeen fish were infected by a monogenean parasite in the gill lamellae. It showed a bilobed anterior extremity, two rows of numerous unequal clamps of microcotylid type, and paired muscular vaginae crowned by differently sized spines. The vaginal number and its relative armature suggested the species’ affiliation to group D; the parasite possessed large, muscular vaginae with a full corona of spines over almost the entire width resembling Bivagina pagrosomi Murray (1931). The molecular analysis of the parasite 28s rRNA revealed 97% homology with B. pagrosomi (AJ577461.1). Conclusion The results confirmed the taxonomic status of the parasite recorded. On the basis of morphology and molecular data, we consider that several conclusions on the systematic status of microcotylids from Red Sea fishes in Saudi Arabia should be discussed.

中文翻译:

Bivagina pagrosomi Murray (1931) (Monogenea: Polyopisthocotylea), 一种感染红海金头鲷鱼鳃的小子叶纲 Sparus aurata (Sparidae):形态和系统发育

摘要 简介 Monogenea 是一类寄生在鱼的皮肤、鳃或鳍上的外寄生扁虫。小子叶科是一个仅寄生于海洋鱼类的多角果叶植物单基因科。这项工作描述并在分类学上确定了沙特水产养殖中一种重要鱼类中的一种微子叶多鱼骨单基因。材料和方法 对从沙特阿拉伯红海捕获的 30 条金头鲷进行了单基因感染检查。在对 28S rRNA 序列进行最大似然分析后,通过光学显微镜对蠕虫进行了形态学和形态计量学描述,并且还构建了多序列比对和系统发育树。结果 17条鱼在鳃片中被单基因寄生虫感染。它显示了一个双叶前肢,两排许多不相等的小子叶型钳,成对的肌肉性阴道,由不同大小的刺加冕。阴道数及其相对骨架表明该物种隶属于 D 组;这种寄生虫拥有大而肌肉发达的阴道,在几乎整个宽度上都有完整的刺冠,类似于 Bivagina pagrosomi Murray (1931)。寄生虫 28s rRNA 的分子分析显示与 B. pagrosomi (AJ577461.1) 有 97% 的同源性。结论 结果证实了记录的寄生虫的分类状况。在形态学和分子数据的基础上,我们认为应该讨论关于沙特阿拉伯红海鱼类微子叶系统状况的几个结论。阴道数及其相对骨架表明该物种隶属于 D 组;这种寄生虫拥有大而肌肉发达的阴道,在几乎整个宽度上都有完整的刺冠,类似于 Bivagina pagrosomi Murray (1931)。寄生虫 28s rRNA 的分子分析显示与 B. pagrosomi (AJ577461.1) 有 97% 的同源性。结论 结果证实了记录的寄生虫的分类状况。在形态学和分子数据的基础上,我们认为应该讨论关于沙特阿拉伯红海鱼类微子叶系统状况的几个结论。阴道数及其相对骨架表明该物种隶属于 D 组;这种寄生虫拥有大而肌肉发达的阴道,在几乎整个宽度上都有完整的刺冠,类似于 Bivagina pagrosomi Murray (1931)。寄生虫 28s rRNA 的分子分析显示与 B. pagrosomi (AJ577461.1) 有 97% 的同源性。结论 结果证实了记录的寄生虫的分类状况。在形态学和分子数据的基础上,我们认为应该讨论关于沙特阿拉伯红海鱼类微子叶系统状况的几个结论。寄生虫 28s rRNA 的分子分析显示与 B. pagrosomi (AJ577461.1) 有 97% 的同源性。结论 结果证实了记录的寄生虫的分类状况。在形态学和分子数据的基础上,我们认为应该讨论关于沙特阿拉伯红海鱼类微子叶系统状况的几个结论。寄生虫 28s rRNA 的分子分析显示与 B. pagrosomi (AJ577461.1) 有 97% 的同源性。结论 结果证实了记录的寄生虫的分类状况。在形态学和分子数据的基础上,我们认为应该讨论关于沙特阿拉伯红海鱼类微子叶系统状况的几个结论。
更新日期:2019-09-13
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