INTRODUCTION Q fever (coxiellosis) is an infectious disease of animals and humans, caused by.C. burnetii and widely distributed throughout the world. It is known that people and animals acquire the disease predominantly.via inhalation of infectious aerosols. The possibility of transmission of the pathogen by the alimentary route is still a matter of debate and remains controversial. Therefore the aim of this study was to fill the gaps in knowledge of oral transmission of.C. burnetii by conducting biological tests on the guinea pig model. MATERIAL AND METHODS Guinea pigs, divided into five groups comprising a negative control and four experimental groups, received specified concentrations of.C. burnetii per os. To determine the presence of specific antibodies, blood samples were tested using CFT. Also, internal organs collected during necropsy were screened by a real-time PCR targeting I.1111. Additionally, histopathological evaluation of the tissues was performed. RESULTS The presence of antibodies and pathogen DNA in caecum was confirmed in one guinea pig from experimental group IV..C. burnetii was also detected in testicular tissue collected from one animal of experimental group II. CONCLUSIONS The presence of pathogen DNA in the testicular tissue indicates that infection spreads haematogenously. In the majority of experimental animals specific antibodies and genetic material of.C. burnetii were not detected. This fact suggests that development of infection depends on many factors, such as animal immune status.

中文翻译：

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在豚鼠模型中通过消化道途径评估 C. Burnetii 传播的可能性。

引言 Q热（coxiellosis）是一种动物和人类的传染病，由.C.引起。burnetii 并广泛分布于世界各地。众所周知，人和动物主要通过吸入传染性气溶胶感染这种疾病。病原体通过消化道传播的可能性仍然存在争议，并且仍然存在争议。因此，本研究的目的是填补口腔传播知识的空白。通过对豚鼠模型进行生物学测试，伯内特氏菌。材料和方法豚鼠，分为五组，包括一个阴性对照和四个实验组，接受特定浓度的C。Burnetii per os。为了确定特定抗体的存在，使用 CFT 测试了血液样本。还，通过针对 I.1111 的实时 PCR 筛选在尸检期间收集的内脏器官。此外，进行了组织的组织病理学评估。结果在实验组IV..C的一只豚鼠中证实了盲肠中存在抗体和病原体DNA。在从实验组 II 的一只动物收集的睾丸组织中也检测到了伯氏杆菌。结论 睾丸组织中病原体 DNA 的存在表明感染是通过血行传播的。在大多数实验动物的特异性抗体和遗传物质中。没有检测到burnetii。这一事实表明，感染的发展取决于许多因素，例如动物的免疫状态。结果在实验组IV..C的一只豚鼠中证实了盲肠中存在抗体和病原体DNA。在从实验组 II 的一只动物收集的睾丸组织中也检测到了伯氏杆菌。结论 睾丸组织中病原体 DNA 的存在表明感染是通过血行传播的。在大多数实验动物的特异性抗体和遗传物质中。没有检测到burnetii。这一事实表明，感染的发展取决于许多因素，例如动物的免疫状态。结果在实验组IV..C的一只豚鼠中证实了盲肠中存在抗体和病原体DNA。在从实验组 II 的一只动物收集的睾丸组织中也检测到了伯氏杆菌。结论 睾丸组织中病原体 DNA 的存在表明感染是通过血行传播的。在大多数实验动物的特异性抗体和遗传物质中。没有检测到burnetii。这一事实表明，感染的发展取决于许多因素，例如动物的免疫状态。结论 睾丸组织中病原体 DNA 的存在表明感染是通过血行传播的。在大多数实验动物的特异性抗体和遗传物质中。没有检测到burnetii。这一事实表明，感染的发展取决于许多因素，例如动物的免疫状态。结论 睾丸组织中病原体 DNA 的存在表明感染是通过血行传播的。在大多数实验动物的特异性抗体和遗传物质中。没有检测到burnetii。这一事实表明，感染的发展取决于许多因素，例如动物的免疫状态。