Accurate histopathological diagnosis is essential for facilitating the optimal surgical management of intracranial germinoma. Current intraoperative histological methods are time- and labor-intensive and often produce artifacts. Multiphoton microscopy (MPM) is a label-free imaging technique that can produce intraoperative histological images of fresh, unprocessed surgical specimens. We employ an MPM based on second-harmonic generation and two-photon excited fluorescence microscopy to image fresh, unfixed, and unstained human germinoma specimens. We show that label-free MPM is not only capable of identifying various cells in human germinoma tissue but also capable of revealing the characteristics of germinoma such as granuloma, stromal fibrosis, calcification, as well as the abnormal and uneven structures of blood vessels. In conjunction with custom-developed image-processing algorithms, MPM can further quantify and characterize the extent of stromal fibrosis and calcification. Our results provide insight into how MPM can deliver rapid diagnostic histological data that could inform the surgical management of intracranial germinoma.

中文翻译：

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使用多光子显微镜快速，无标签地检测颅内生殖瘤。

准确的组织病理学诊断对于促进颅内生殖瘤的最佳外科治疗至关重要。当前的术中组织学方法是费时费力的并且经常产生伪像。多光子显微镜（MPM）是一种无标记的成像技术，可以产生新鲜的，未经处理的手术标本的术中组织学图像。我们采用基于第二谐波产生和双光子激发荧光显微镜的MPM来成像新鲜的，未固定的和未染色的人类生殖瘤样本。我们表明，无标签的MPM不仅能够识别人类生殖细胞瘤组织中的各种细胞，而且能够揭示生殖细胞瘤的特征，例如肉芽肿，间质纤维化，钙化以及血管的异常和不均匀结构。结合定制开发的图像处理算法，MPM可以进一步量化和表征基质纤维化和钙化的程度。我们的结果提供了关于MPM如何提供快速诊断组织学数据的见识，这些数据可以为颅内发芽瘤的外科治疗提供依据。