In the post-genomic era, gene function analysis has attracted much attention. Transformation is often needed to investigate gene function. In this study, an easy, rapid, reliable, and cost-effective colony polymerase chain reaction (PCR) method for screening mushroom transformants was developed: picking up a suitable amount of transformant’s tissue (1–10 μg) to 20 μl 0.25% Lywallzyme solution, and vortexing for 10 s followed by incubation at 34 °C for 15 min. Finally, 2 μl of the suspension was used as templates to perform PCR and single target bands were successfully amplified from respective transformants of Tremella fuciformis, Pleurotus ostreatus, and Pleurotus tuber-regium. This procedure could be widely employed for screening transformants in mushroom transformation experiments.

在后基因组时代，基因功能分析引起了人们的广泛关注。通常需要进行转化以研究基因功能。在这项研究中，开发了一种简便，快速，可靠且具有成本效益的菌落聚合酶链反应（PCR）方法来筛选蘑菇转化子：将适量的转化子组织（1–10μg）提取到20μl0.25％Lywallzyme溶液，涡旋10 s，然后在34°C孵育15分钟。最后，将2μl悬浮液用作模板进行PCR，并成功地从银耳，平菇和侧柏的各个转化体中成功扩增了单个靶条带。该方法可广泛用于蘑菇转化实验中筛选转化子。