Endophytic fungi have the ability to live inside the host plant tissues without causing neither symptoms of diseases/or harm. Opportunistic infections are accountable for majority of the outbreaks, thereby putting a burden on the health system. To investigate and characterize the bioactive compounds for the control of bacteria of clinical importance, extracts from endophytic fungi were isolated from indigenous South African medicinal plants. Extracts from endophytic fungi were isolated from 133 fungal strains and screened against Gram positive and negative bacteria namely Bacillus cereus, Escherichia coli, Enterococcus faecium, and E. gallinarum using disk diffusion. Furthermore, gas chromatography–mass spectrometry was performed to identify the bioactive compounds. Sixteen out of one hundred and thirty-three (12%) fungi extracts exhibited antibacterial properties against some of the selected bacteria. E. coli was found to be the most susceptible in contrast to E. faecium and E. gallinarum which were the most resistant. The isolate MHE 68, identified as Alternaria sp. displayed the greater spectrum of antibacterial activities by controlling selected clinical bacteria strains including resistant E. faecium and E. gallinarum. The chemical analysis of the extract from MHE 68 indicated that linoleic acid (9,12-octadecadienoic acid (Z,Z)) and cyclodecasiloxane could be accountable for the antibacterial activity. This is the first study conducted on the secondary metabolites produced by endophytic fungal strains isolated from the Pelargonium sidoides DC. possessing antibacterial properties.

内生真菌具有生活在宿主植物组织内的能力，而不会引起疾病/危害的症状。机会性感染是大多数暴发的原因，因此给卫生系统造成了负担。为了研究和表征用于控制具有临床重要性的细菌的生物活性化合物，从南非土著药用植物中分离出了内生真菌的提取物。从133种真菌菌株中分离到内生真菌的提取物，并对革兰氏阳性细菌和阴性细菌（蜡状芽孢杆菌，大肠杆菌，粪肠球菌和鸡大肠杆菌）进行筛选。使用磁盘扩散。此外，进行了气相色谱-质谱法鉴定生物活性化合物。一百三十三（12％）真菌提取物中有十六种表现出对某些选定细菌的抗菌性能。与抵抗力最强的粪肠球菌和鸡毒肠球菌相比，大肠杆菌是最易感的。分离株MHE 68，鉴定为链格孢菌。通过控制选定的临床细菌菌株（包括耐药性屎肠球菌和鸡肠球菌）显示出更大的抗菌活性谱。对MHE 68提取物的化学分析表明，亚油酸（9,12-十八碳二烯酸（Z，Z））和环十硅氧烷可能是造成抗菌活性的原因。这是对从天竺葵中提取的内生真菌菌株产生的次级代谢产物进行的首次研究。具有抗菌性能。