Streptomyces blastmyceticus strain 12-6 was isolated from a forest soil sample of Cheonan area on the basis of strong antifungal activities against plant pathogenic fungi. Butanol extracts of the cultural filtrates were active against C. acutatum, C. coccodes, C. gloeosporioides, F. oxysporum, and T. roseum. Active fractions were prepared by thin layer chromatography using silica gel plate; 12-6-2 (R_f 0.36), 12-6-3 (R_f 0.44). Scanning electron microscopy showed that the active fractions caused a change in surface texture of fungal spores from smooth surface to wrinkled surface. The lethal effect on the spores of the active fractions varied from 56% to 100%. It was shown that the spores of C. acutatum were more sensitive to the antifungal fractions than the spores of F. oxysporum. Fluorescence staining using TOTO-1 indicated that the antifungal fractions could make the spores more sensitive to the fluorescence dye. Thus, it was suggested that antifungal agents prepared in this study exhibited the antifungal activity by damaging the plasma membrane of both fungal spores and hyphae. Identification of antifungal agents in the active fraction using GC-MS analysis revealed the presence of cyclo-(Leu-Pro) and 9-octadecenamide as major components that have already been known as antifungal substances.

基于对植物病原真菌的强抗真菌活性，从天安地区的森林土壤样品中分离出了Streptomyces blastmyceticus菌株12-6。文化滤液的丁醇提取物是有效对抗C. acutatum，C. coccodes，炭疽菌，尖孢镰刀菌和T.霉。用硅胶板通过薄层色谱法制备活性级分；12-6-2（R _f 0.36），12-6-3（R _f0.44）。扫描电子显微镜显示，活性级分引起真菌孢子表面结构从光滑表面到起皱表面的变化。活性成分对孢子的致死作用从56％到100％不等。结果表明，与尖孢镰刀菌的孢子相比，硬壳梭菌的孢子对抗真菌成分更敏感。使用TOTO-1的荧光染色表明，抗真菌组分可以使孢子对荧光染料更敏感。因此，表明本研究中制备的抗真菌剂通过破坏真菌孢子和菌丝的质膜而显示出抗真菌活性。使用GC-MS分析鉴定活性级分中的抗真菌剂表明，存在环-（Leu-Pro）和9-十八烯酰胺是已被称为抗真菌物质的主要成分。