Bio-sulfur can be produced in the process of desulfurization from a landfill and collected by some microorganism such as Thiobacillus sp. as a sulfur element. In order to investigate practical use of bio-sulfur as an agent for controlling plant disease, in vitro antifungal activity of bio-sulfur was tested against Colletotrichum orbiculare known to cause cucumber anthracnose. Efficacy of bio-sulfur for suppressing anthracnose disease was also evaluated in vivo using cucumber leaves. Mycelial growth of C. orbiculare on medium containing bio-sulfur was inhibited. Disease severity of cucumber leaves pre-treated with bio-sulfur was significantly decreased compared to that of untreated ones. To illustrate how bio-sulfur could suppress anthracnose disease, structures of cucumber leaves infected with C. orbiculare were observed under a fluorescent microscope and a scanning electron microscope (SEM). Cucumber leaves pre-treated with bio-sulfur showed a low rate of appressorium formation whereas untreated ones showed abundant appressoria. Shrunk fungal hyphae were mostly observed on bio-sulfur-pretreated leaves by SEM. Similar results were observed on leaves pre-treated with a commercial fungicide Benomyl®. These results suggest that inhibition of appressorium formation of C. orbiculare by bio-sulfur may contribute to its suppression of cucumber anthracnose.

生物硫可以在垃圾填埋场的脱硫过程中产生，并被某些微生物（如硫杆菌属）收集。作为硫元素。为了研究生物硫作为控制植物病害的试剂的实际用途，测试了生物硫在体外对已知引起黄瓜炭疽病的球形炭疽菌的抗真菌活性。还使用黄瓜叶片在体内评估了生物硫抑制炭疽病的功效。的菌丝体生长C.炭疽病在含有生物硫的培养基上的抑制作用。与未处理相比，经生物硫预处理的黄瓜叶片的病害严重程度明显降低。为了说明生物硫如何抑制炭疽病，在荧光显微镜和扫描电子显微镜（SEM）下观察了感染了圆球菌的黄瓜叶片的结构。经生物硫预处理的黄瓜叶片的表皮形成率低，而未处理的叶片则具有丰富的表皮。SEM观察到在经过生物硫预处理的叶片上大部分观察到了真菌菌丝的收缩。在用商业杀菌剂Benomyl®预处理的叶子上观察到了相似的结果。这些结果表明，抑制了梭状芽胞杆菌的顶壁形成。 通过生物硫可能有助于其抑制黄瓜炭疽病。