Ferritin, an iron-binding protein, is composed of two subunits, a heavy chain and a light chain. It regulates many biological functions, such as proliferation, angiogenesis, and immunosuppression. The objective of this study was to determine the expression and distribution of ferritin in the periodontal tissuesof primates.First, we assessed the expression of ferritin in primary cultured cells isolated from human periodontal tissues using the polymerase chain reaction and immunofluorescent staining. Second, we investigated the expression and distribution of ferritin in the periodontal tissues of Macaca fascicularis, human gingival tissues, and human gingival carcinoma tissues using immunohistochemistry.Both protein and mRNA of ferritin were constitutively present in human primary cultured cells, including those from the dental apical papilla, periodontal ligament, dental pulp, and gingival epithelium, as well as gingival fibroblasts. In M. fascicularistissues, the immunohistochemical staining was particularly strong in blood vessel and mineralizing areas of the dental pulp and periodontal ligament. Ferritin heavy chain exhibited specific immunopositivity in in the stratum basale of the epithelium in human gingival tissue and strong immunostaining was found in peripheral regions of gingival carcinoma sites. Ferritin is constitutivelypresent andwidelydistributed in the periodontal tissues of primates. Ferritin may play roles in epithelial proliferation, vascular angiogenesis, and mineralization in these tissues.

中文翻译：

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铁蛋白在灵长类动物的牙周组织中的表达。

铁蛋白是一种铁结合蛋白，由两个亚基组成：重链和轻链。它调节许多生物学功能，例如增殖，血管生成和免疫抑制。这项研究的目的是确定铁蛋白在灵长类动物牙周组织中的表达和分布。首先，我们使用聚合酶链反应和免疫荧光染色评估了从人牙周组织分离的原代培养细胞中铁蛋白的表达。其次，我们采用免疫组织化学方法研究了猕猴，人牙龈组织和人牙龈癌组织中铁蛋白的表达和分布，其中铁蛋白的蛋白和mRNA均组成性地存在于人原代培养细胞中，包括来自牙齿的细胞。顶端乳头 牙周膜，牙髓，牙龈上皮以及牙龈成纤维细胞。在束状分枝杆菌中，免疫组织化学染色在牙髓和牙周膜的血管和矿化区域特别强。铁蛋白重链在人牙龈组织中上皮的基底层中表现出特异性的免疫阳性，并且在牙龈癌部位的周围区域发现强的免疫染色。铁蛋白在灵长类动物的牙周组织中组成性分布并广泛分布。铁蛋白可能在这些组织中的上皮增殖，血管血管生成和矿化中发挥作用。免疫组织化学染色在牙髓和牙周膜的血管和矿化区域特别强。铁蛋白重链在人牙龈组织中上皮的基底层中表现出特异性的免疫阳性，并且在牙龈癌部位的周围区域发现强的免疫染色。铁蛋白在灵长类动物的牙周组织中组成性分布并广泛分布。铁蛋白可能在这些组织中的上皮增殖，血管血管生成和矿化中发挥作用。免疫组织化学染色在牙髓和牙周膜的血管和矿化区域特别强。铁蛋白重链在人牙龈组织中上皮的基底层中表现出特异性的免疫阳性，并且在牙龈癌部位的周围区域发现强的免疫染色。铁蛋白在灵长类动物的牙周组织中组成性分布并广泛分布。铁蛋白可能在这些组织中的上皮增殖，血管血管生成和矿化中发挥作用。铁蛋白在灵长类动物的牙周组织中组成性分布并广泛分布。铁蛋白可能在这些组织中的上皮增殖，血管血管生成和矿化中发挥作用。铁蛋白在灵长类动物的牙周组织中组成性分布并广泛分布。铁蛋白可能在这些组织中的上皮增殖，血管血管生成和矿化中发挥作用。