The mechanism of action of treatment of either curcumin or capsaicin or in combination on LPS (Lipopolysaccharide) induced inflammatory gene expression in peripheral blood mononuclear cells (PBMCs) was investigated using RT-PCR and in silico docking methods. RT-PCR analysis has shown that the curcumin and capsaicin significantly reduced LPS induced over expression of COX-2, IL-6 and TGF-β in PBMCs. Whereas combined molecules demonstrated synergistic response on the reduction of COX-2, IL-6 and TGF-β over expression in LPS induced PBMCs as compared to individual molecules. Further, The docking of curcumin and capsaicin at the active pockets of COX-2, IL-6 and TGF-β has shown - 3.90, - 4.49 and - 5.61 kcal/mol binding energy for curcumin and - 3.80, - 4.78 and - 5.76 kcal/mol binding energy for capsaicin, while multiple ligand simultaneous docking (MLSD) of both molecules has shown higher binding energy of - 4.24, - 5.35 and - 5.83 kcal/mol respectively. This has demonstrated the efficacy of combined curcumin and capsaicin against the LPS induced expression of pro-inflammatory cytokines in PBMCs. These results attributed the coordinated positive modulation on biochemical and molecular cellular process by combined curcumin and capsaicin as compared to individual molecules.

中文翻译：

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姜黄素和辣椒素调节人外周血单个核细胞中LPS诱导的COX-2，IL-6和TGF-β的表达。

使用RT-PCR和计算机对接方法研究了姜黄素或辣椒素或其组合对LPS（脂多糖）诱导的外周血单核细胞（PBMC）中炎症基因表达的作用机理。RT-PCR分析表明，姜黄素和辣椒素显着降低了PBMCs中COX-2，IL-6和TGF-β的过度表达所诱导的LPS。而结合的分子与LPS诱导的PBMC相比，在LPS诱导的PBMC中表现出协同减少COX-2，IL-6和TGF-β的协同反应。此外，姜黄素和辣椒素在COX-2，IL-6和TGF-β的活性口袋中的对接显示，姜黄素的结合能为-3.90，-4.49和-5.61 kcal / mol，而-3.80，-4.78和-5.76辣椒素的千卡/摩尔结合能，而两个分子的多个配体同时对接（MLSD）已显示出更高的结合能，分别为-4.24，-5.35和-5.83 kcal / mol。这已经证明姜黄素和辣椒素联合使用可抵抗LPS诱导的PBMC中促炎性细胞因子的表达。这些结果归因于姜黄素和辣椒素与单个分子相比，对生化和分子细胞过程的协同正调控。