Phage display antibody libraries have proven an invaluable resource for the isolation of diagnostic and potentially therapeutic antibodies, the latter usually being antibody fragments converted into IgG formats. Recent advances in the production of highly diverse and functional antibody libraries are considered here, including for Fabs, scFvs and nanobodies. These advances include codon optimisation during generation of CDR diversity, improved display levels using novel signal sequences, molecular chaperones and isomerases and the use of highly stable scaffolds with relatively high expression levels. In addition, novel strategies for the batch reformatting of scFv and Fab phagemid libraries, derived from phage panning, into IgG formats are described. These strategies allow the screening of antibodies in the end-use format, facilitating more efficient selection of potential therapeutics.

中文翻译：

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噬菌体抗体文库的生产和批量重新格式化的进展。

噬菌体展示抗体库已被证明是分离诊断和潜在治疗性抗体的宝贵资源，后者通常是转化为IgG格式的抗体片段。本文考虑了高度多样化和功能性抗体库生产的最新进展，包括Fab，scFv和纳米抗体。这些进展包括在CDR多样性产生过程中的密码子优化，使用新型信号序列，分子伴侣和异构酶改善的显示水平以及使用具有相对高表达水平的高度稳定的支架。另外，描述了将来自噬菌体淘选的scFv和Fab噬菌粒文库批量重新格式化为IgG形式的新策略。这些策略可以筛选最终用途形式的抗体，