Sugar kelp (Saccharina latissima) is an economically important species, and natural populations provide diverse and productive habitats as well as important ecosystem services. For seaweed aquaculture to be successful in newly emerging industry in Europe and other Western countries, it will have to develop sustainable production management strategies. A key feature in this process is the capacity to conserve genetic diversity for breeding programs aimed at developing seed stock for onward cultivation, as well as in the management of wild populations, as potentially interesting genetic resources are predicted to disappear due to climate change. In this study, the cryopreservation of male and female gametophytes (haploid life stage) of S. latissima by different combinations of two-step cooling methods and cryoprotectants was explored. We report here that cryopreservation constitutes an attractive option for the long-term preservation of S. latissima gametophytes, with viable cells in all treatment combinations. The highest viabilities for both male and female gametophytes were found using controlled-rate cooling methods combined with dimethyl sulfoxide 10% (v/v). Morphological normal sporophytes were observed to develop from cryopreserved vegetative gametophytic cells, independent of treatment. This indicates that cryopreservation is a useful preservation method for male and female S. latissima gametophytes.

中文翻译：

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支持海藻生物技术的发展：褐藻（Saccharina latissima）配子体的冷冻保存。

糖海带 (Saccharina latissima) 是一种重要的经济物种，自然种群提供多样化和富有成效的栖息地以及重要的生态系统服务。海藻养殖要想在欧洲和其他西方国家的新兴产业中取得成功，就必须制定可持续的生产管理战略。这一过程的一个关键特征是能够保护育种计划的遗传多样性，这些育种计划旨在开发种子库以供进一步种植，以及管理野生种群，因为预计潜在的有趣遗传资源将因气候变化而消失。本研究探索了通过两步冷却方法和冷冻保护剂的不同组合来冷冻保存宽阔子草雄性和雌性配子体（单倍体生命阶段）。我们在此报告，冷冻保存是长期保存宽阔阔叶藻配子体的一个有吸引力的选择，在所有治疗组合中都具有活细胞。使用控制速率冷却方法结合 10% (v/v) 二甲基亚砜发现雄性和雌性配子体的最高活力。观察到形态正常的孢子体由冷冻保存的营养配子体细胞发育而来，与处理无关。这表明冷冻保存是宽阔阔叶藻雄性和雌性配子体的一种有用的保存方法。