Primary cilia, hair-like organelles projecting from the surface of cells, are critical for sensing extracellular stimuli and transmitting molecular signals that regulate cell functions. During bone development, cell cilia are found in several types of cells, but their roles require further investigation. Intraflagellar transport (IFT) is essential for the formation and maintenance of most eukaryotic cilia. IFT140 is a core protein of the IFT-A complex. Mutations in IFT140 have been associated with cases of skeletal ciliopathies. In this study, we examined the expression of IFT140 during bone development. The results showed that, compared with many soft tissues, Ift140 (mRNA level) was highly expressed in bone. Moreover, its expression level was downregulated in the long bones of murine osteoporosis models. At the histological level, IFT140 was characteristically expressed in osteoblasts and chondrocytes at representative stages of bone development, and its expression level in these two types of cells was observed in two waves. These findings suggest that IFT140 may play an important role in the process of chondrogenic and osteogenic differentiation during bone development.

中文翻译：

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IFT140在骨发育过程中的表达。

从细胞表面突出的原发性纤毛，像头发一样的细胞器，对于感知细胞外刺激并传递调节细胞功能的分子信号至关重要。在骨骼发育过程中，在几种类型的细胞中都发现了细胞纤毛，但是它们的作用需要进一步研究。鞭毛内运输（IFT）对于大多数真核纤毛的形成和维持至关重要。IFT140是IFT-A复合物的核心蛋白。IFT140的突变与骨骼肌纤毛病有关。在这项研究中，我们检查了IFT140在骨骼发育过程中的表达。结果表明，与许多软组织相比，Ift140（mRNA水平）在骨中高表达。此外，其表达水平在鼠骨质疏松模型的长骨中被下调。在组织学层面上，IFT140在骨骼发育的代表性阶段在成骨细胞和软骨细胞中具有特征性表达，并且在两种波中观察到了它在这两种类型的细胞中的表达水平。这些发现表明，IFT140可能在骨骼发育过程中的软骨形成和成骨分化过程中发挥重要作用。