The tissue microarray (TMA) is a powerful tool for cancer biomarker discovery and validation. Tens to hundreds of samples can be evaluated simultaneously for molecular marker status at the protein or nucleic acid level. Although fully automated or semiautomated technologies for TMA creation provide excellent precision with respect to core transfer, they do not obviate the need for technical expertise to successfully generate high-quality TMA blocks and derivative sections. We have leveraged our expanding bank of formalin-fixed paraffin embedded paired tumor and normal tissues in our academic cancer center to provide a rich source of input material for cancer research TMAs. In this study, we report a stepwise optimization of TMA generation parameters, including paraffin wax selection, tempering protocol, and sectioning conditions, to achieve the best ribbon sectioning.

中文翻译：

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在癌症研究环境中，从人类福尔马林固定石蜡包埋的组织中组织微阵列的优化。

组织微阵列（TMA）是用于癌症生物标记物发现和验证的强大工具。可以同时评估数十到数百个样品的蛋白质或核酸水平上的分子标记状态。尽管用于TMA创建的全自动或半自动化技术在堆芯转移方面提供了卓越的精度，但是它们并没有消除成功生成高质量TMA块和派生段所需的技术知识。我们已利用我们在学术癌症中心扩大的福尔马林固定石蜡包埋的配对肿瘤和正常组织库来为癌症研究TMA提供丰富的输入资料来源。在这项研究中，我们报告了TMA生成参数的逐步优化，包括石蜡的选择，回火方案和切片条件，