The present study was conducted to identify the differentially expressed miRNAs (DE miRNAs) in the peripheral blood mononuclear cells of crossbred pigs in response to CSF vaccination on 7 and 21 days of post vaccination as compared to unvaccinated control (0 dpv). Simultaneously, set of miRNA was predicted using mRNA seq data at same time point. The proportion of CD4⁻CD8⁺ and CD4⁺CD8⁺ increased after vaccination, and the mean percentage inhibition was 86.89% at 21 dpv. It was observed that 22 miRNAs were commonly expressed on both the time points. Out of predicted DE miRNAs, it was found that 40 and 35 DE miRNAs were common, obtained from miRNA seq analysis and predicted using mRNA seq data on 7 dpv versus 0 dpv and 21 dpv versus 0 dpv respectively. Two DE miRNAs, ssc-miR-22-5p and ssc-miR-27b-5p, were selected based on their log₂ fold change and functions of their target genes in immune process/pathway of viral infections. The validations of DE miRNAs using qRT-PCR were in concordance with miRNA seq analysis. Two set of target genes, CD40 and SWAP70 (target gene of ssc-miR-22-5p) and TLR4 and Lyn (target gene of ssc-miR-27b-5p), were validated and were in concordance with results of RNA seq analysis at a particular time point (except TLR4). The first report of genome-wide identification of differentially expressed miRNA in response to live attenuated vaccine virus of classical swine fever revealed miR-22-5p and miR-27b-5p were differentially expressed at 7 dpv and 21 dpv.

中文翻译：

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对 miRNA 和 mRNA 表达谱进行全基因组整合分析，以鉴定响应经典猪瘟疫苗病毒的差异表达的 miR-22-5p 和 miR-27b-5p。

进行本研究以确定与未接种疫苗的对照（0 dpv）相比，杂交猪在接种疫苗后第 7 天和第 21 天响应 CSF 疫苗接种的外周血单核细胞中差异表达的 miRNA（DE miRNA）。同时，使用同一时间点的 mRNA seq 数据预测一组 miRNA。^{CD4 −} CD8 ⁺和 CD4 ⁺ CD8 ⁺的比例疫苗接种后增加，21 dpv 时的平均抑制百分比为 86.89%。据观察，22 个 miRNA 在两个时间点上普遍表达。在预测的 DE miRNA 中，发现 40 和 35 个 DE miRNA 是常见的，它们是从 miRNA seq 分析中获得的，并使用分别在 7 dpv 与 0 dpv 和 21 dpv 与 0 dpv 上的 mRNA seq 数据进行预测。两种 DE miRNA，ssc-miR-22-5p 和 ssc-miR-27b-5p，是根据它们的 log ₂倍变化和它们的靶基因在病毒感染的免疫过程/途径中的功能来选择的。使用 qRT-PCR 的 DE miRNA 验证与 miRNA seq 分析一致。两组靶基因，CD40和SWAP70（ssc-miR-22-5p的靶基因）和TLR4和Lyn（ssc-miR-27b-5p 的目标基因），经过验证并与特定时间点的 RNA seq 分析结果一致（TLR4 除外）。响应经典猪瘟减毒活疫苗病毒的差异表达 miRNA 全基因组鉴定的第一份报告显示，miR-22-5p 和 miR-27b-5p 在 7 dpv 和 21 dpv 时差异表达。