A variety of hematological diseases manifest in the bone marrow (BM), broadly characterized as BM failure (BMF). BMF can be caused by acute lymphoblastic leukemia (ALL), which results in an expansion of hypoxic regions in the BM. Because of this hypoxic presentation, there is potential for improved characterization of BMF through in vivo assessment of oxygenation in the BM cavity. Photoacoustic (PA) imaging can provide local assessment of intravascular oxygen saturation (SO₂), which has been shown to correlate with pimonidazole-assessed hypoxia. This study introduces an optimized PA imaging technique to assess SO₂ within the femoral BM cavity through disease progression in a murine model of ALL. Results show a statistically significant difference with temporal changes in SO₂ (from baseline) between control and diseased cohorts, demonstrating the potential of PA imaging for noninvasive, label-free monitoring of BMF diseases.

骨髓（BM）表现出多种血液疾病，广泛地被称为BM衰竭（BMF）。BMF可由急性淋巴细胞白血病（ALL）引起，导致BM中缺氧区域的扩大。由于这种低氧表现，有可能通过体内评估BM腔内的氧合来改善BMF的表征。光声（PA）成像可提供血管内血氧饱和度（SO ₂）的局部评估，这已显示与吡莫尼唑评估的缺氧相关。这项研究引入了一种优化的PA成像技术来评估SO ₂在ALL小鼠模型中通过疾病进展在股骨BM腔内转移。结果显示，对照组和患病人群之间SO ₂（相对于基线）的时间变化具有统计学上的显着差异，表明PA成像对BMF疾病进行无创，无标签监测的潜力。