The interaction of proteins with implantable metallic surfaces has a great influence on the bioactivity and biodegradation of orthopaedic implants. Initial osseointegration is known to be critical for the long term success of orthopaedic implants. The surface properties of the implant and electrochemical milieu of the surrounding solution such as electrostatic, hydrophobic, and hydrogen bonding interactions significantly modulate protein adsorption by implants. Magnesium (Mg) is considered to improve the adhesion of osteoblasts via ligand binding of the integrin receptors. Mg-based composites, reinforced with hydroxyapatite (HA), are potential candidates for temporary orthopaedic implants. However, their clinical translation requires enhanced degradation resistance in physiological environment so that it is in sync with the healing rate of the bone. The present study deals with the protein adsorption characteristics and degradation behaviour of Mg-HA-based biodegradable implants. Quantitative analysis of apatite inducing ability of composites was evaluated in terms of mass gain in simulated body fluid (SBF) as well as in foetal bovine serum (FBS), by an in vitro immersion study. Incorporation of 5 and 15 wt% HA to Mg-3Zn improved apatite formation up to 35% and 66%, respectively, after 14 days of immersion in SBF. Compared to FBS, SBF is found to be significantly more effective in precipitating apatite on a Mg-HA surface. However, FBS offered more corrosion resistance to Mg-HA than SBF did, as evident from the significant differences in the protein adhesion capabilities of the composite surface when incubated separately in these two mediums. The addition of 15 wt% HA enhanced the protein adsorption capability by ∼35%. These studies highlight the possibility of modulating the degradation and bioactivity of Mg-based composite by tailoring the composition of HA. These findings, in turn, warrant the suitability of Mg-HA composite in orthopaedic application.

中文翻译：

---

火花等离子体烧结制备的镁基临时骨科植入物在血清和模拟体液中的差异体外降解和蛋白质粘附行为。

蛋白质与可植入金属表面的相互作用对整形外科植入物的生物活性和生物降解有很大影响。众所周知，初始骨整合对于骨科植入物的长期成功至关重要。植入物的表面特性和周围溶液的电化学环境（例如静电，疏水和氢键相互作用）显着调节植入物对蛋白质的吸附。镁（Mg）被认为可通过整联蛋白受体的配体结合来改善成骨细胞的粘附性。以羟基磷灰石（HA）增强的镁基复合材料是临时骨科植入物的潜在候选材料。然而，他们的临床翻译要求在生理环境中增强抗降解性，使其与骨骼的愈合速度同步。本研究涉及基于Mg-HA的可生物降解植入物的蛋白质吸附特性和降解行为。通过体外浸没研究，通过模拟体液（SBF）和胎牛血清（FBS）的质量增加来评估复合材料磷灰石诱导能力的定量分析。在SBF中浸泡14天后，将5wt％和15 wt％的HA掺入Mg-3Zn可使磷灰石的形成分别提高35％和66％。与FBS相比，发现SBF在Mg-HA表面沉淀磷灰石方面更为有效。但是，与SBF相比，FBS对Mg-HA的耐腐蚀性更高，从分别在这两种介质中孵育时复合材料表面的蛋白质粘附能力的显着差异可以明显看出这一点。添加15 wt％的HA可将蛋白质吸附能力提高35％。这些研究突出了通过调整HA的组成来调节Mg基复合材料降解和生物活性的可能性。这些发现反过来保证了Mg-HA复合材料在整形外科中的适用性。