Infection by the castrating parasitic nematode Sphaerularia bombi changes gene expression in Bombus terrestris bumblebee queens.,Insect Molecular Biology

当前位置： X-MOL 学术 › Insect Mol. Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Infection by the castrating parasitic nematode Sphaerularia bombi changes gene expression in Bombus terrestris bumblebee queens.
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2019-10-21 , DOI: 10.1111/imb.12618
T J Colgan _{1,

2,

3} , J C Carolan ₄ , S Sumner ₅ , M L Blaxter ₆ , M J F Brown ₇

Affiliation

Parasitism can result in dramatic changes in host phenotype, which are themselves underpinned by genes and their expression. Understanding how hosts respond at the molecular level to parasites can therefore reveal the molecular architecture of an altered host phenotype. The entomoparasitic nematode Sphaerularia bombi is a parasite of bumblebee (Bombus) hosts where it induces complex behavioural changes and host castration. To examine this interaction at the molecular level, we performed genome‐wide transcriptional profiling using RNA‐Sequencing (RNA‐Seq) of S. bombi‐infected Bombus terrestris queens at two critical time‐points: during and just after overwintering diapause. We found that infection by S. bombi affects the transcription of genes underlying host biological processes associated with energy usage, translation, and circadian rhythm. We also found that the parasite affects the expression of immune genes, including members of the Toll signalling pathway providing evidence for a novel interaction between the parasite and the host immune response. Taken together, our results identify host biological processes and genes affected by an entomoparasitic nematode providing the first steps towards a molecular understanding of this ecologically important host–parasite interaction.

中文翻译：

the割性寄生线虫Sphaerularia bombi的感染改变了Bombus terrestris Bumblebee Queen中的基因表达。

寄生可能导致宿主表型发生巨大变化，而宿主表型本身受基因及其表达的支持。因此，了解宿主如何在分子水平上对寄生虫作出反应可以揭示宿主表型改变的分子结构。昆虫寄生线虫Sphaerularia bombi是大黄蜂（Bombus）寄主的寄生虫，它会诱发复杂的行为变化和寄主去势。为了在分子水平上检查这种相互作用，我们使用S的RNA测序（RNA-Seq）进行了全基因组转录谱分析。在两个关键的时间点被炸弹感染的Bombus terrestris皇后：越冬期和滞育后不久。我们发现小号。庞比影响与能量使用，翻译和昼夜节律有关的宿主生物过程的基因转录。我们还发现，该寄生虫影响免疫基因的表达，包括Toll信号通路的成员，为寄生虫与宿主免疫反应之间的新型相互作用提供了证据。综上所述，我们的研究结果确定了受昆虫寄生线虫影响的宿主生物过程和基因，为分子生物学理解这种具有重要生态意义的宿主-寄生虫相互作用提供了第一步。

更新日期：2019-10-21

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11