In this study, the anticancer activities of two siRNA carriers were compared using a human lung adenocarcinoma epithelial cell line (A549). Firstly, poly(styrene)-graft-poly(linoleic acid) (PS-g-PLina) and poly(styrene)-graft-poly(linoleic acid)-graft-poly(ethylene glycol) (PS-g-PLina-g-PEG) graft copolymers were synthesized by free-radical polymerization. PS-PLina and PS-PLina-PEG nanoparticles (NPs) were prepared by solvent evaporation method and were then characterized. The size was found as 150 ± 10 nm for PS-PLina and 184 ± 6 nm for PS-PLina-PEG NPs. The NPs were functionalized with poly(l-lysine) (PLL) for c-myc siRNA conjugation. siRNA entrapment efficiencies were found in the range of 4–63% for PS-PLina-PLL and 6–42% for PS-PLina-PEG-PLL NPs. The short-term stability test was realised for 1 month. siRNA release profiles were also investigated. In vitro anticancer activity of siRNA-NPs was determined by MTT, flow cytometry, and fluorescence microscopy analyses. Obtained findings showed that both NPs systems were promising as siRNA delivery tool for lung cancer therapy.

在这项研究中，使用人肺腺癌上皮细胞系（A549）比较了两种siRNA载体的抗癌活性。首先，聚（苯乙烯）-接枝-聚（亚油酸）（PS- g - PLina-）和聚（苯乙烯）-接枝-聚（亚油酸）-接枝-聚乙二醇（PS- g - PLina- g-PEG）接枝共聚物是通过自由基聚合合成的。通过溶剂蒸发法制备了PS-PLina和PS-PLina-PEG纳米颗粒（NPs），并对其进行了表征。对于PS-PLina，发现大小为150±10 nm，对于PS-PLina-PEG NP，发现大小为184±6 nm。将NP用聚（1-赖氨酸）（PLL）官能化以用于c-myc siRNA缀合。对于PS-PLina-PLL而言，发现siRNA的捕获效率范围为4–63％，对于PS-PLina-PEG-PLL NP，发现的范围为6–42％。短期稳定性测试实现了1个月。还研究了siRNA的释放曲线。通过MTT，流式细胞仪和荧光显微镜分析确定了siRNA-NPs的体外抗癌活性。获得的发现表明，两种NPs系统都有望作为用于肺癌治疗的siRNA传递工具。