In insects, nutrition and hormones coordinately regulate lifespan and reproduction, which might affect each other. We here investigated how nutrition, insulin, and juvenile hormone (JH) signal genes affect lifespan and reproduction in the German cockroach, Blattella germanica, a serious urban pest throughout the world. Starvation as well as altering insulin and nutrition signal genes by RNA interference (RNAi) knockdown of the expression of either positive or negative components in the two pathways simultaneously reduced lifespan and ootheca number of the mated female cockroaches. Meanwhile, depletion of the JH receptor Methoprene-tolerant (Met), but not kruppel homolog 1 (Kr-h1) that predominately transduces JH signaling to prevent metamorphosis, significantly reduced the two parameters. Moreover, suppressing the expression of several reproduction-related genes, including doublesex (Dsx), vitellogenin (Vg), and the Vg receptor (VgR), had little yet various effects on lifespan; nevertheless, it is likely that there are some reproduction-independent mating factors reducing lifespan. In conclusion, through blocking lifespan and reproduction in a simultaneous manner, the alteration of insulin and nutrient signal gene expression or the depletion of Met might provide a great potential for controlling the German cockroach.

中文翻译：

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胰岛素和营养信号基因表达的改变或Met的消耗会减少德国蟑螂的寿命和繁殖。

在昆虫中，营养和激素协调调节寿命和繁殖，这可能会相互影响。我们在这里研究了营养，胰岛素和少年激素（JH）信号基因如何影响德国蟑螂德国小Bl（Blattella germanica）的寿命和繁殖，而德国小Bl是全世界的一种严重城市害虫。饥饿以及通过RNA干扰（RNAi）敲低两条途径中阳性或阴性组分的表达而改变胰岛素和营养信号基因，同时降低了交配雌性蟑螂的寿命和卵囊数。同时，JH受体甲氨蝶呤耐受（Met）的耗竭，但主要转导JH信号传导以防止变态的kruppel同系物1（Kr-h1）却没有，这两个参数显着降低。此外，抑制几个与生殖有关的基因的表达，包括双性（Dsx），卵黄蛋白原（Vg）和Vg受体（VgR），对寿命几乎没有多方面的影响；但是，很可能存在一些与繁殖无关的交配因子会缩短寿命。总之，通过同时阻断寿命和繁殖，胰岛素和营养信号基因表达的改变或Met的消耗可能为控制德国蟑螂提供了巨大的潜力。