Five chimney swift fledglings died following a progressive loss of appetite and condition while being cared for by an experienced wildlife rehabilitator. All animals had severe necrotizing and heterophilic ventriculitis, with myriad epithelial cells characterized by karyomegaly with intranuclear inclusion bodies. Transmission electron microscopy showed distention of epithelial cell nuclei and chromatin peripheralization by nonenveloped, icosahedral, 75- to 85-nm-diameter virions. Degenerate nested PCR for a highly conserved region of the adenovirus DNA polymerase gene was positive. BLAST analysis of the amplicon sequence indicated the presence of a novel adenovirus, with 74% homology to Antarctic penguin adenoviruses and 72% homology to a bat adenovirus, at low query coverages of only 65% and 63%, respectively. BLAST analysis of the predicted amino acid sequence generated the highest scores for squamate adenoviruses at 100% query coverage. Based on phylogenetic analysis of the partial amino acid sequence of the DNA polymerase, the chimney swift virus was a novel adenovirus most closely related to the Atadenovirus genus. Using a probe based on the novel viral sequence, DNA in situ hybridization identified viral nucleic acid in the nucleus. While the tentatively named chimney swift adenovirus-1 (CsAdV-1) is so far classified with the Atadenoviruses, it is relatively divergent from other members of that genus and may represent the first identified member of a new genus of Adenoviruses.

中文翻译：

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与新型腺病毒，烟囱雨燕腺病毒-1（CsAdV-1）相关的雏鸟烟囱雨燕（Chaetura Pelagica）中的坏死性心室炎。

五只烟囱幼雏由于食欲和条件的逐渐丧失而死亡，同时有经验的野生动物康复者对其进行照顾。所有动物均患有严重的坏死性和嗜异性心室炎，其无数的上皮细胞具有核内包涵体的核富集特征。透射电子显微镜显示直径为75到85 nm的二十面体非包膜病毒粒子使上皮细胞核膨胀，并使染色质外围化。腺病毒DNA聚合酶基因高度保守区域的简并嵌套式PCR为阳性。对扩增子序列的BLAST分析表明，存在新的腺病毒，其与南极企鹅腺病毒具有74％的同源性，与蝙蝠腺病毒具有72％的同源性，在低查询覆盖率下分别仅为65％和63％。预测的氨基酸序列的BLAST分析在100％查询覆盖率下产生了鳞状腺病毒的最高分。根据DNA聚合酶部分氨基酸序列的系统发育分析，烟囱迅速病毒是一种与腺病毒属最密切相关的新型腺病毒。使用基于新型病毒序列的探针，DNA原位杂交可在细胞核中鉴定出病毒核酸。到目前为止，暂定名为烟囱快速腺病毒1（CsAdV-1）的是腺病毒，但它与该属的其他成员相对不同，可能代表了腺病毒新属的第一个鉴定成员。根据DNA聚合酶部分氨基酸序列的系统发育分析，烟囱迅速病毒是一种与腺病毒属最密切相关的新型腺病毒。使用基于新型病毒序列的探针，DNA原位杂交可在细胞核中鉴定出病毒核酸。到目前为止，暂定名为烟囱快速腺病毒1（CsAdV-1）的是腺病毒，但它与该属的其他成员相对不同，并且可能代表了腺病毒新属的第一个鉴定成员。根据DNA聚合酶部分氨基酸序列的系统发育分析，烟囱迅速病毒是一种与腺病毒属最密切相关的新型腺病毒。使用基于新型病毒序列的探针，DNA原位杂交可在细胞核中鉴定出病毒核酸。到目前为止，暂定名为烟囱快速腺病毒1（CsAdV-1）的是腺病毒，但它与该属的其他成员相对不同，并且可能代表了腺病毒新属的第一个鉴定成员。