In humans and in mouse models, precursor B-cell lymphoblastic leukemia (B-ALL)/lymphoblastic lymphoma (B-LBL) can be classified as either the pro-B or pre-B subtype. This is based on the expression of antigens associated with the pro-B and pre-B stages of B-cell development. Antigenic markers can be detected by flow cytometry or immunohistochemistry (IHC), but no comparison of results from these techniques has been reported for murine B-ALL/LBL. In our analysis of 30 cases induced by chemical or viral mutagenesis on a WT or Pax5+/- background, 18 (60%) were diagnosed as pro-B by both flow cytometry and IHC. Discordant results were found for 12 (40%); 6 were designated pro-B by IHC and pre-B by flow cytometry and the reverse for the remaining 6 cases. Discordance occurred because different markers were used to define the pro-B-to-pre-B transition by IHC vs flow cytometry. IHC expression of cytoplasmic IgM (μIgM) defined the pre-B stage, whereas the common practice of using CD25 as a surrogate marker in flow cytometry was employed here. These results show that CD25 and μIgM are not always concurrently expressed in B-ALL/LBL, in contrast to normal B-cell development. Therefore, when subtyping B-ALL/LBL in mice, an IHC panel of B220, PAX5, TdT, c-Kit/CD117, CD43, IgM, and ΚLC should be considered. For flow cytometry, cytoplasmic IgM may be an appropriate marker in conjunction with the surface markers B220, CD19, CD43, c-Kit/CD117, BP-1, and CD25.

中文翻译：

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小鼠前体 B 细胞白血病/淋巴瘤的免疫表型分析：免疫组织化学和流式细胞术的比较。


在人类和小鼠模型中，前体 B 细胞淋巴母细胞白血病 (B-ALL)/淋巴母细胞淋巴瘤 (B-LBL) 可分为前 B 亚型或前 B 亚型。这是基于与 B 细胞发育的 pro-B 和 pre-B 阶段相关的抗原表达。抗原标记可通过流式细胞术或免疫组织化学 (IHC) 进行检测，但尚未报道这些技术对小鼠 B-ALL/LBL 的结果进行比较。我们对 WT 或 Pax5+/- 背景下化学或病毒诱变诱导的 30 例病例进行分析，其中 18 例 (60%) 通过流式细胞术和 IHC 诊断为 pro-B。 12 人（40%）的结果不一致； 6 例通过 IHC 被指定为 pro-B，通过流式细胞仪被指定为 pre-B，其余 6 例则相反。出现不一致的原因是 IHC 与流式细胞术使用不同的标记来定义 pro-B 到 pre-B 的转变。细胞质 IgM (μIgM) 的 IHC 表达定义了前 B 阶段，而此处采用了在流式细胞术中使用 CD25 作为替代标记的常见做法。这些结果表明，与正常 B 细胞发育相反，CD25 和 μIgM 在 B-ALL/LBL 中并不总是同时表达。因此，在对小鼠进行 B-ALL/LBL 分型时，应考虑 B220、PAX5、TdT、c-Kit/CD117、CD43、IgM 和 KLC 的 IHC 组合。对于流式细胞术，细胞质 IgM 可能是与表面标记 B220、CD19、CD43、c-Kit/CD117、BP-1 和 CD25 结合的合适标记。