当前位置: X-MOL 学术Appl. Immunohistochem. Mol. Morphol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Culture Cell Block Controls as a Tool to the Biomolecular Diagnosis of Infectious Diseases
Applied Immunohistochemistry & Molecular Morphology ( IF 1.3 ) Pub Date : 2019-10-17 , DOI: 10.1097/pai.0000000000000811
Leonardo José Tadeu de Araujo 1 , David Salas-Gómez 1 , Lidia Midori Kimura 1 , Juliana Fernandes Possatto Takahashi 1 , Joana de Souza Barrel 1 , Dominique C Rollin 2 , Juliana Mariotti Guerra 1
Affiliation  

The cell block (CB) technique has allowed easy obtainment of samples such as cellular and culture suspensions, to perform specific molecular tests such as immunohistochemistry and in situ hybridization. It has been improved along time, accuracy, and quality of the diagnoses, however, the cost of a commercial gel matrix for the preparation of CB is high and not suitable depending on the situation. The objective of this study is to test agarose as an alternative to the commercial gel matrix in the preparation of Aspergillus fumigatus’ CB.

中文翻译:

培养细胞块控制作为传染病生物分子诊断的工具

细胞块 (CB) 技术可以轻松获取细胞和培养悬液等样品,以进行特定的分子测试,例如免疫组织化学和原位杂交。随着时间的推移,诊断的准确性和质量得到了提高,但是,用于制备 CB 的商业凝胶基质成本高,不适合根据情况。本研究的目的是测试琼脂糖作为商业凝胶基质的替代品,用于制备烟曲霉的 CB。
更新日期:2019-10-17
down
wechat
bug