RNA interference-mediated knockdown of the transcription factor Krüppel homologue 1 suppresses vitellogenesis in Chilo suppressalis.,Insect Molecular Biology

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RNA interference-mediated knockdown of the transcription factor Krüppel homologue 1 suppresses vitellogenesis in Chilo suppressalis.
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2019-10-21 , DOI: 10.1111/imb.12617
Y Tang ₁ , H He ₁ , X Qu ₁ , Y Cai ₁ , W Ding _{2,

3} , L Qiu ₁ , Y Li _{1,

2}

Affiliation

Vitellogenesis in holometabolous insects involves the production and secretion of vitellogenin (Vg) and other yolk protein precursors in developing oocyte by the fat body, all of which is predominantly orchestrated by juvenile hormone (JH). Krüppel homologue 1 (Kr‐h1) is a zinc finger transcription factor that has been demonstrated to be a JH‐early inducible gene and to contribute to reproduction. However, the exact molecular function of Kr‐h1 in insect reproduction is poorly understood. In the current study, we used the notorious pest Chilo suppressalis as a model system to investigate the role of Kr‐h1 in female reproduction. Cloning and sequencing C. suppressalis Kr‐h1 revealed that it shares high identity with its homologues from other lepidopteran insects. Moreover, RNA interference‐mediated knockdown of CsKr‐h1 substantially reduced the transcription of Vg in the fat body, dramatically decreased yolk protein deposition and also impaired oocyte maturation and ovarian development, indicating that Kr‐h1 is indispensable for normal vitellogenesis in C. suppressalis. Based on these results, we conclude that Kr‐h1 is crucial to reproduction in insects and that targeting this gene could potentially be a new way to suppress rice pests.

中文翻译：

RNA干扰介导的转录因子Krüppel同源物1的敲低抑制了Chilo inhibitoralis中的卵黄发生。

同源代谢昆虫的卵黄发生涉及脂肪体在卵母细胞发育过程中产生和分泌卵黄蛋白原（Vg）和其他卵黄蛋白前体，所有这些主要由幼体激素（JH）精心组织。Krüppel同系物1（Kr-h1）是锌指转录因子，已被证明是JH早期可诱导的基因，并有助于生殖。但是，人们对Kr-h1在昆虫繁殖中的确切分子功能知之甚少。在当前的研究中，我们使用臭名昭著的害虫Chilo inhibitoralis作为模型系统来研究Kr-h1在雌性生殖中的作用。克隆和测序隐球菌Kr-h1揭示它与其他鳞翅目昆虫的同系物具有高度同一性。此外，RNA干扰介导的CsKr-h1的敲低显着降低了脂肪体内Vg的转录，显着降低了卵黄蛋白的沉积，还损害了卵母细胞的成熟和卵巢的发育，这表明Kr-h1对于抑制隐孢子虫的正常卵黄形成是必不可少的。。根据这些结果，我们得出结论，Kr-h1对昆虫繁殖至关重要，而靶向该基因可能是抑制水稻害虫的新途径。

更新日期：2019-10-21

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