Background: Aflatoxins are one of the most heavily regulated mycotoxins in agriculture throughout the world. A variety of tests are used for detection, including rapid methods that are preferred when a large number of samples need to be quickly screened to implement an immediate action. However, a method developed for screening a specific commodity for the presence of mycotoxins requires further validation to demonstrate its suitability for additional matrices. Objective: In this study, a study was undertaken to evaluate a rapid screening method for aflatoxin B1 (AFB1) in dry dog food, a product potentially susceptible to aflatoxins contamination. Method: This test method employed lateral flow technology using kits obtained from Charm Sciences Inc. Three different sources of dry dog food were tested at the FDA action level of 20 ppb (ng/g) in three trials of a multi-laboratory study by four participants. A total of 80 unfortified blank samples, 270 samples spiked at 20 ppb, and 60 samples spiked below 20 ppb were analyzed. Results: The overall pass rates of 100% for unfortified samples and > 97% for 20 ppb-fortified samples meet the FDA guidance acceptance criteria for a limit test of 10-15% false positives and no more than 5% false negatives. Conclusions: The method is suitable for screening a large number of dry dog food samples for rapid response. Highlights: Multi-laboratory evaluation of a rapid method for aflatoxin screening in dog food.

中文翻译：

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用于干狗粮中黄曲霉毒素B1分析的侧向流动方法的多实验室评估。

背景：黄曲霉毒素是全世界农业中最严格的霉菌毒素之一。多种测试可用于检测，包括快速方法，当需要快速筛选大量样本以立即采取行动时，首选快速方法。但是，为筛选特定商品中是否存在霉菌毒素而开发的方法需要进一步验证，以证明其适用于其他基质。目的：在这项研究中，进行了一项研究，以评估干狗粮中黄曲霉毒素B1（AFB1）的快速筛选方法，该产品可能易受黄曲霉毒素污染。方法：该测试方法使用了从Charm Sciences Inc.获得的试剂盒的侧向流动技术。在四名参与者进行的多实验室研究的三项试验中，以FDA作用水平20 ppb（ng / g）测试了三种不同来源的干狗粮。共分析了80个未强化的空白样品，270个加标为20 ppb的样品和60个加标为20 ppb以下的样品。结果：未强化样品的总体合格率为100％，而20 ppb强化样品的总体合格率为> 97％，符合FDA指导接受标准，即假阳性率为10-15％，假阴性不超过5％。结论：该方法适用于大量干狗粮样品的快速反应筛查。强调： 多实验室评估一种狗粮中黄曲霉毒素的快速筛选方法。