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NeoSeekTM STEC: A Multiplex Molecular Method for Detection and Identification of Select Shiga Toxin-Producing Escherichia coli in Beef.
Journal of AOAC INTERNATIONAL ( IF 1.7 ) Pub Date : 2020-04-20 , DOI: 10.5740/jaoacint.19-0300
Edan Hosking 1 , Brooke Roman 1 , Susan Alles 1 , Mark Mozola 1 , Susanne Hinkley 2 , Karen Cooper 2 , Danielle Keys 2 , Benjamin Bastin 3 , Wesley Thompson 3 , Robert Donofrio 1 , Yi Chen 4 , Maria Cristina Fernandez 5 , Michael Brodsky 6
Affiliation  

Background: NeoSeekTM STEC is a single-source, service-based method for detection and identification of select Shiga toxin–producing Escherichia coli (STEC), including E. coli O157:H7 and STEC of somatic groups O26, O45, O103, O111, O121, and O145. The method is a multiplex molecular method utilizing more than 80 genetic targets to identify STEC in complex matrices such as food enrichment cultures. Objective: A study was conducted to validate the NeoSeek method for detection of select STEC in raw beef trim. Methods: Performance of the NeoSeek STEC method was compared with that of the U.S. Department of Agriculture, Food Safety and Inspection Service reference methods for E. coli O157:H7 and non-O157 STEC for detection of E. coli O157:H7 and E. coli O26:H11 in raw beef trim. Additionally, inclusivity/exclusivity testing and method robustness testing were performed. Results: Results of raw beef trim testing showed no statistically significant differences in performance between the NeoSeek and reference methods in the ability to detect either E. coli O157:H7 or E.coli O26:H11, as determined by probability of detection analysis. Results of inclusivity and exclusivity testing showed 100% expected results with target and nontarget bacteria, with the exception of a single strain of E. coli O157:H7, which was subsequently verified to be stx-negative by PCR. Conclusions and Highlights: NeoSeek STEC is an accurate, reliable method for rapid detection and identification of select STEC in complex populations such as beef trim enrichment cultures.

中文翻译:

NeoSeekTM STEC:一种检测和鉴定牛肉中产生志贺毒素的大肠杆菌的多重分子方法。

背景: NeoSeek TM STEC是一种基于服务的单一来源的方法,用于检测和鉴定某些产志贺毒素的大肠杆菌(STEC),包括大肠杆菌O157:H7和体细胞O26,O45,O103,O111的STEC。 ,O121和O145。该方法是一种多重分子方法,利用80多个遗传靶标来鉴定复杂基质(例如食物富集培养)中的STEC。目的:进行了一项研究,以验证NeoSeek方法在生牛肉条中检测特定STEC的方法。方法:将NeoSeek STEC方法的性能与美国农业部,食品安全和检验服务局的大肠杆菌参考方法进行比较O157:H7和非O157 STEC用于检测生牛肉条中的大肠杆菌O157:H7和大肠杆菌O26:H11。此外,还进行了包容性/排他性测试和方法稳健性测试。结果:生牛肉切边试验的结果表明,通过检测概率分析,NeoSeek和参考方法在检测大肠杆菌O157:H7或大肠杆菌O26:H11的能力上在性能上无统计学差异。包容性和排他性测试的结果表明,针对靶细菌和非靶细菌的预期结果为100%,唯一的大肠杆菌O157:H7菌株除外,该菌株随后被证实为stx-PCR阴性。结论和亮点: NeoSeek STEC是一种准确,可靠的方法,可用于快速检测和鉴定复杂人群(如牛肉切边培养)中的精选STEC。
更新日期:2020-04-20
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