In decapod crustaceans, the amines dopamine, octopamine, serotonin, and histamine are known to serve as locally released and/or circulating neuromodulators. While many studies have focused on determining the modulatory actions of amines on decapod nervous systems, comparatively little is known about the identity of the receptors through which they exert their actions. Here, a crayfish, Procambarus clarkii, tissue-specific transcriptome was used to identify putative amine receptors in the eyestalk, a structure composed largely of the eyestalk ganglia, including the neuroendocrine X-organ-sinus gland system, and retina. Transcripts encoding 17 distinct putative amine receptors, three dopamine (one dopamine 1-like, one dopamine 2-like, and one dopamine/ecdysteroid-like), five octopamine (one alpha-like, three beta-like, and one octopamine/tyramine-like), three serotonin (two type-1-like and one type-7-like), and six histamine (five histamine-gated chloride channel A-like and one histamine-gated chloride channel B-like) were identified in the assembly. Comparison of the nucleotide sequence of the transcript encoding one predicted type-1-like serotonin receptor with that cloned previously from the P. clarkii nervous system shows the two sequences to be essentially identical, providing increased support for the validity of the transcripts used to deduce the proteins reported here. Reciprocal BLAST and structural/functional domain analyses support the protein family annotations ascribed to the putative P. clarkii receptors. These data represent the first large-scale description of amine receptors from P. clarkii, and as such provide a new resource for initiating gene-based studies of aminergic control of physiology/behavior at the level of receptors in this species.

在十足目甲壳类动物中，已知胺类多巴胺、章鱼胺、血清素和组胺可作为局部释放和/或循环的神经调节剂。虽然许多研究都集中在确定胺对十足动物神经系统的调节作用，但人们对它们发挥作用的受体的身份知之甚少。在这里，小龙虾（克氏原螯虾）的组织特异性转录组被用来识别眼柄中假定的胺受体，眼柄主要由眼柄神经节组成，包括神经内分泌 X 器官-窦腺系统和视网膜。编码 17 种不同的假定胺受体、三种多巴胺（一种多巴胺 1 样、一种多巴胺 2 样和一种多巴胺/蜕皮类固醇样）、五种章鱼胺（一种 α 类、三种 β 类和一种章鱼胺/酪胺）的转录本中鉴定出三种血清素（两种 1 型样和一种 7 型样）和六种组胺（五种组胺门控氯通道 A 样和一种组胺门控氯通道 B 样）。集会。将编码一种预测的 1 型样血清素受体的转录本的核苷酸序列与之前从克氏疟原虫神经系统中克隆的转录本的核苷酸序列进行比较，表明这两个序列基本上是相同的，这为用于推断的转录本的有效性提供了更多的支持。此处报道的蛋白质。相互 BLAST 和结构/功能域分析支持归因于假定的克氏疟原虫受体的蛋白质家族注释。这些数据代表了对克氏疟原虫胺受体的首次大规模描述，因此为启动该物种受体水平上生理/行为的胺能控制的基于基因的研究提供了新的资源。