Altered miRNA expression participates in the biological progress of thyroid carcinoma and functions as a diagnostic marker or therapeutic agent. However, the role of miR-7-2-3p is currently unclear. The authors' study was the first investigation of miR-7-2-3p expression level and diagnostic ability in several public databases. Potential target genes were obtained from DIANA Tools, and function enrichment analysis was then performed. Furthermore, the authors examined expression levels of potential targets in the Human Protein Atlas (HPA) and the Cancer Genome Atlas (TCGA). Finally, the potential transcription factors (TFs) were predicted by JASPAR. TCGA, GSE62054, GSE73182, GSE40807, and GSE55780 revealed that miR-7-2-3p expression in papillary thyroid carcinoma (PTC) tissues was notably lower compared with non-tumour tissues, while its expression in E-MATB-736 showed no remarkable difference. Function enrichment analysis showed that 698 genes were enriched in pathways, including pathways in cancer, and glioma. CCND1, GSK3B, and ITGAV of pathways in cancer were inverse correlations with miR-7-2-3p in both post-transcription and protein levels. According to the TF prediction, the prospective upstream TFs of miR-7-2-3p were ISX, SPI1, PRRX1, and BARX1. MiR-7-2-3p was significantly down-regulated and may act on PTC progression by crucial pathways. However, the mechanisms of miR-7-2-3p need further investigation.

中文翻译：

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多种检测方法探讨甲状腺乳头状癌中miR-7-2-3p的下调及其临床意义


改变的 miRNA 表达参与甲状腺癌的生物学进展，并作为诊断标志物或治疗剂发挥作用。然而，miR-7-2-3p 的作用目前尚不清楚。作者的研究是对几个公共数据库中 miR-7-2-3p 表达水平和诊断能力的首次调查。从DIANA Tools中获取潜在的靶基因，然后进行功能富集分析。此外，作者还检查了人类蛋白质图谱 (HPA) 和癌症基因组图谱 (TCGA) 中潜在靶标的表达水平。最后，JASPAR 预测了潜在的转录因子（TF）。 TCGA、GSE62054、GSE73182、GSE40807和GSE55780显示，与非肿瘤组织相比，甲状腺乳头状癌（PTC）组织中miR-7-2-3p的表达量显着降低，而E-MATB-736中的表达量则无显着差异。不同之处。功能富集分析显示，698个基因在通路中富集，包括癌症和神经胶质瘤中的通路。癌症通路中的 CCND1、GSK3B 和 ITGAV 在转录后和蛋白水平上均与 miR-7-2-3p 呈负相关。根据TF预测，miR-7-2-3p的预期上游TF是ISX、SPI1、PRRX1和BARX1。 MiR-7-2-3p 显着下调，可能通过关键途径影响 PTC 进展。然而，miR-7-2-3p的机制需要进一步研究。