当前位置：
X-MOL 学术
›
Insect Mol. Biol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Silencing of the DNA methyltransferase 1 associated protein 1 (DMAP1) gene in the invasive ladybird Harmonia axyridis implies a role of the DNA methyltransferase 1-DMAP1 complex in female fecundity.
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2019-10-17 , DOI: 10.1111/imb.12616 J Gegner 1 , T Gegner 1, 2 , H Vogel 3 , A Vilcinskas 1, 2
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2019-10-17 , DOI: 10.1111/imb.12616 J Gegner 1 , T Gegner 1, 2 , H Vogel 3 , A Vilcinskas 1, 2
Affiliation
|
The invasive harlequin ladybird Harmonia axyridis is a textbook example of polymorphism and polyphenism as the temperature during egg development determines the frequency of melanic morphs and the number and size of black spots in nonmelanic morphs. Recent concepts in evolutionary biology suggest that epigenetic mechanisms can translate environmental stimuli into heritable phenotypic changes. To investigate whether epigenetic mechanisms influence the penetrance and expressivity of colour morphs in H. axyridis, we used RNA interference to silence key enzymes required for DNA methylation and histone modification. We found that neither of these epigenetic mechanisms affected the frequency of different morphs, but there was a significant impact on life‐history traits such as longevity and fecundity. Strikingly, we found that silencing the gene encoding for DNA methyltransferase 1 associated protein 1 (DMAP1) severely reduced female fecundity, which correlated with an abundance of degenerated ovaries in DMAP1‐knockdown female beetles. Finally, we observed significant differences in DMAP1 expression when we compared native and invasive H. axyridis populations with a biocontrol strain differing in egg‐laying capacity, suggesting that the DNA methyltransferase 1‐DMAP1 complex may influence the invasive performance of this ladybird.
中文翻译：
DNA甲基转移酶1相关蛋白1（DMAP1）基因在入侵瓢虫Harmonia axyridis中的沉默暗示了DNA甲基转移酶1-DMAP1复合体在女性生殖力中的作用。
侵袭性丑角瓢虫异色瓢虫是多态性与多型的教科书例如作为卵子发育过程中的温度确定黑色素变种和在nonmelanic变种的黑点的数目和大小的频率。进化生物学的最新概念表明，表观遗传机制可以将环境刺激转化为可遗传的表型变化。调查表观遗传机制是否影响轴心菌颜色形态的渗透性和表达能力，我们使用RNA干扰来沉默DNA甲基化和组蛋白修饰所需的关键酶。我们发现，这些表观遗传机制均未影响不同形态的频率，但对寿命和生育力等生活史特征产生了重大影响。令人惊讶的是，我们发现沉默编码DNA甲基转移酶1相关蛋白1（DMAP1）的基因会严重降低雌性繁殖力，这与DMAP1敲除雌性甲虫中大量退化的卵巢有关。最后，我们观察到在显著差异DMAP1当我们比较天然和侵入性表达异色瓢虫 生物控制菌株的产卵能力不同，这表明DNA甲基转移酶1-DMAP1复合物可能会影响该瓢虫的侵袭性能。
更新日期：2019-10-17
中文翻译：
DNA甲基转移酶1相关蛋白1（DMAP1）基因在入侵瓢虫Harmonia axyridis中的沉默暗示了DNA甲基转移酶1-DMAP1复合体在女性生殖力中的作用。
侵袭性丑角瓢虫异色瓢虫是多态性与多型的教科书例如作为卵子发育过程中的温度确定黑色素变种和在nonmelanic变种的黑点的数目和大小的频率。进化生物学的最新概念表明，表观遗传机制可以将环境刺激转化为可遗传的表型变化。调查表观遗传机制是否影响轴心菌颜色形态的渗透性和表达能力，我们使用RNA干扰来沉默DNA甲基化和组蛋白修饰所需的关键酶。我们发现，这些表观遗传机制均未影响不同形态的频率，但对寿命和生育力等生活史特征产生了重大影响。令人惊讶的是，我们发现沉默编码DNA甲基转移酶1相关蛋白1（DMAP1）的基因会严重降低雌性繁殖力，这与DMAP1敲除雌性甲虫中大量退化的卵巢有关。最后，我们观察到在显著差异DMAP1当我们比较天然和侵入性表达异色瓢虫 生物控制菌株的产卵能力不同，这表明DNA甲基转移酶1-DMAP1复合物可能会影响该瓢虫的侵袭性能。
京公网安备 11010802027423号