We provide a transcriptional profile of coffee rust interaction and identified putative up regulated resistant genes Coffee rust disease, caused by the fungus Hemileia vastatrix, is one of the major diseases in coffee throughout the world. The use of resistant cultivars is considered to be the most effective control strategy for this disease. To identify candidate genes related to different mechanism defense in coffee, we present a time-course comparative gene expression profile of Caturra (susceptible) and Híbrido de Timor (HdT, resistant) in response to H. vastatrix race XXXIII infection. The main objectives were to obtain a global overview of transcriptome in both interaction, compatible and incompatible, and, specially, analyze up-regulated HdT specific genes with inducible resistant and defense signaling pathways. Using both Coffea canephora as a reference genome and de novo assembly, we obtained 43,159 transcripts. At early infection events (12 and 24 h after infection), HdT responded to the attack of H. vastatrix with a larger number of up-regulated genes than Caturra, which was related to prehaustorial resistance. The genes found in HdT at early hours were involved in receptor-like kinases, response ion fluxes, production of reactive oxygen species, protein phosphorylation, ethylene biosynthesis and callose deposition. We selected 13 up-regulated HdT-exclusive genes to validate by real-time qPCR, which most of them confirmed their higher expression in HdT than in Caturra at early stage of infection. These genes have the potential to assist the development of new coffee rust control strategies. Collectively, our results provide understanding of expression profiles in coffee-H. vastatrix interaction over a time course in susceptible and resistant coffee plants.

中文翻译：

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咖啡的高通量转录组分析揭示了对 Hemileia mastatrix 感染的反应前的抗性。

我们提供了咖啡锈病相互作用的转录谱并确定了推定的上调抗性基因咖啡锈病是由真菌 Hemileia mastatrix 引起的，是全世界咖啡中的主要疾病之一。使用抗性品种被认为是该病害最有效的控制策略。为了鉴定与咖啡中不同机制防御相关的候选基因，我们提出了 Caturra（易感）和 Híbrido de Timor（HdT，抗性）响应 H.vastatrix 种族 XXXIII 感染的时程比较基因表达谱。主要目标是在相互作用、相容和不相容方面获得转录组的全球概览，特别是分析具有诱导抗性和防御信号通路的上调 HdT 特异性基因。使用 Coffea canephora 作为参考基因组和从头组装，我们获得了 43,159 个转录本。在早期感染事件中（感染后 12 和 24 小时），HdT 以比 Caturra 更多的上调基因对 H. mastatrix 的攻击作出反应，这与前吸音阻力有关。早期在 HdT 中发现的基因与受体样激酶、反应离子通量、活性氧的产生、蛋白质磷酸化、乙烯生物合成和胼胝质沉积有关。我们选择了 13 个上调的 HdT 专有基因通过实时 qPCR 进行验证，其中大多数证实了它们在感染早期在 HdT 中的表达高于在 Caturra 中的表达。这些基因有可能帮助开发新的咖啡锈病控制策略。集体，我们的结果提供了对咖啡-H 中表达谱的理解。易感和抗性咖啡植物在一段时间内的vasatrix相互作用。